| Autor: |
John L. VandeBerg, James G. Fox, Paul B. Samollow, Yan Feng, Calvin Ho, Zeli Shen, Briony D Smith, Vince Gresham, Anthony Mannion, Maggie Lin, Stephanie Yang, Vasudevan Bakthavatchalu, James J Elliott, Michael Esmail |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Int J Syst Evol Microbiol |
| Popis: |
In a search for potential causes of increased prolapse incidence in grey short-tailed opossum colonies, samples from the gastrointestinal tracts of 94 clinically normal opossums with rectal prolapses were screened forHelicobacterspecies by culture and PCR. Forty strains of two novelHelicobacterspecies which differed from the establishedHelicobactertaxa were isolated from opossums with and without prolapses. One of theHelicobacterspecies was spiral-shaped and urease-negative whereas the otherHelicobacterstrain had fusiform morphology with periplasmic fibres and was urease-positive. 16S rRNA gene sequence analysis revealed that all the isolates had over 99 % sequence identity with each other, and were most closely related toHelicobacter canadensis. Strains from the two novelHelicobacterspecies were subjected togyrBandhsp60gene and whole genome sequence analyses. These two novelHelicobacterspecies formed separate phylogenetic clades, divergent from other knownHelicobacterspecies. The bacteria were confirmed as novelHelicobacterspecies based on digital DNA–DNA hybridization and average nucleotide identity analysis of their genomes, for which we propose the namesHelicobacter monodelphidissp. nov. with the type strain MIT 15-1451T(=LMG 29780T=NCTC 14189T) andHelicobacter didelphidarumsp. nov with type strain MIT 17-337T(=LMG 31024T=NCTC 14188T) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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