Murine B7 antigen provides an efficient costimulatory signal for activation of murine T lymphocytes via the T-cell receptor/CD3 complex
| Autor: | Ziba Razi-Wolf, Hans Reiser, Claude D. Gimmi, Gordon J. Freeman, Baruj Benacerraf, Lee M. Nadler |
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| Rok vydání: | 1992 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes Interleukin 2 CD3 Complex Glycosylphosphatidylinositols CD3 Receptors Antigen T-Cell CHO Cells Lymphocyte Activation Phosphatidylinositols Transfection Mice Antigen Cricetinae medicine Animals Antigens Ly Receptor Calcimycin Multidisciplinary biology Chinese hamster ovary cell T-cell receptor Receptors Interleukin-2 T lymphocyte Blotting Northern Molecular biology Up-Regulation Antigens Surface B7-1 Antigen biology.protein Interleukin-2 Tetradecanoylphorbol Acetate Thy-1 Antigens Glycolipids Research Article Signal Transduction medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 89:271-275 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | We demonstrate that the murine B7 (mB7) protein is a potent costimulatory molecule for the activation of resting murine CD4+ T cells through the T-cell receptor (TCR)/CD3 complex. Stable mB7-transfected Chinese hamster ovary cells, but not vector-transfected controls, synergize with anti-CD3 monoclonal antibody and Con A-induced T-cell activation, resulting ultimately in proliferation. mB7 exerted its effect by inducing production of interleukin 2 and expression of the interleukin 2 receptor. Thus, mB7 costimulates T-cell activation through the TCR/CD3 complex by positively modulating the normal pathway of T-cell expansion. In contrast to the pronounced effect of mB7 on the activation of T cells through the TCR/CD3 complex, the mB7-transfected CHO cell line costimulated T-cell activation via the glycosylphosphatidylinositol-anchored proteins Thy-1 and Ly-6A.2 only inefficiently. Finally, the combination of a calcium ionophore and mB7 is not sufficient to cause T-cell proliferation, while the combination of a calcium ionophore and phorbol 12-myristate 13-acetate (PMA) stimulates T cells efficiently. The signals that mB7 and PMA provide for murine T lymphocyte activation are therefore not interchangeable, although both costimulate activation through the TCR/CD3 complex. |
| Databáze: | OpenAIRE |
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