A conventional polymerase chain reaction-based method for the diagnosis of human schistosomiasis in stool samples from individuals in a low-endemicity area
| Autor: | Sara Menezes de Oliveira, Marta Cristhiany Cunha Pinheiro, José Mauro Peralta, Fernando Schemelzer de Moraes Bezerra, Regina Helena Saramago Peralta, Teiliane Rodrigues Carneiro |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Adult Veterinary medicine lcsh:Arctic medicine. Tropical medicine Adolescent Kato-Katz lcsh:RC955-962 lcsh:QR1-502 Schistosomiasis saline gradient Biology Polymerase Chain Reaction Sensitivity and Specificity lcsh:Microbiology law.invention Feces Young Adult law parasitic diseases medicine Animals Humans Child Parasite Egg Count Polymerase chain reaction Aged Helmintex ® Articles Schistosoma mansoni DNA Helminth Middle Aged biology.organism_classification medicine.disease Schistosomiasis mansoni PCR Child Preschool Kato katz Immunology ELISA Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 108, Iss 8, Pp 1037-1044 (2013) Memórias do Instituto Oswaldo Cruz, Volume: 108, Issue: 8, Pages: 1037-1044, Published: 06 DEC 2013 Memórias do Instituto Oswaldo Cruz |
| ISSN: | 1678-8060 |
| Popis: | The aim of this study was to evaluate the efficacy of a polymerase chain reaction (PCR)-based method to detect Schistosoma mansoni DNA in stool samples from individuals living in a low-endemicity area in Brazil. Of the 125 initial stool samples, 80 were ELISA reactive and eggs were identified in 19 of the samples by parasitological examination. For the PCR evaluations, 56 stool samples were selected and divided into five groups. Groups I-IV were scored negative for S. mansoni eggs by parasitological examination. Groups I and II were ELISA reactive, whereas Groups III and IV were ELISA nonreactive. Groups II and III were positive for other intestinal parasites. PCR testing scored eight samples as positive from these four groups. Group V represented the S. mansoni -positive group and it included ELISA-reactive samples that were scored positive for S. mansoni by one or more parasitological examinations (6/19 were positive by Kato-Katz method, 9/17 by saline gradient and 10/13 by Helmintex®). PCR scored 13 of these 19 samples as positive for S. mansoni . We conclude that while none of these methods yielded 100% sensitivity, a combination of techniques should be effective for improving the detection of S. mansoni infection in low-endemicity areas. |
| Databáze: | OpenAIRE |
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