Study of the alteration of gene expression in adipose tissue of diet-induced obese mice by microarray and reverse transcription-polymerase chain reaction analyses
| Autor: | A Blondet, Torben F. Ørntoft, K Birkenkamp-Demtroeder, J. Tirard, Ricardo C. Moraes, Danielle Naville, Philippe Durand, Martine Begeot, Arieh Gertler |
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| Přispěvatelé: | ProdInra, Migration, Communications Cellulaires et Différenciation (CCD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Blood Glucose
Leptin Male medicine.medical_specialty Gene Expression Adipose tissue White adipose tissue Biology Mice chemistry.chemical_compound Endocrinology Adipocyte Internal medicine Genetic model medicine Animals Insulin Obesity OLIGO NUCLEOTIDE Oligonucleotide Array Sequence Analysis [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism Leptin receptor Reverse Transcriptase Polymerase Chain Reaction Microarray analysis techniques Gene Expression Profiling Body Weight [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism Dietary Fats SIGNAL Up-Regulation Mice Inbred C57BL Adipose Tissue chemistry Corticosterone Diet-induced obese |
| Zdroj: | Endocrinology Endocrinology, Endocrine Society, 2003, 144 (11), pp.4773-4782 Moraes, R C, Blondet, A, Birkenkamp-Demtröder, K, Tirard, J, Ørntoft, T F, Gertler, A, Durand, P, Naville, D & Begeot, M 2003, ' Study of the alteration of gene expression in adipose tissue of diet-induce obese mice by microarray and reverse transcription-polymerase chain reaction analyses ', Endocinology, vol. 144, no. 11, pp. 4773-4782 . Aarhus University |
| ISSN: | 0013-7227 |
| Popis: | In the present study we developed a model of diet-induced obesity (DIO) in male C57 BL/6J mice using an 8-wk high fat diet. This model should better reflect the physiology of the majority of the human obese patients than mouse genetic models of obesity with defects in leptin or leptin signaling. At the end of the diet, DIO mice displayed an increased weight (20%) and higher leptin, insulin, glucose, and corticosterone plasma levels compared with mice fed a standard diet during the same period. Moreover, they became resistant to the central effect of peripheral administration of leptin. Oligonucleotide microarray studies were conducted in adipose tissue. They showed that a great number of genes are differentially expressed. The majority of these genes (69%) are down-regulated in DIO mice. Among those are genes encoding enzymes of the lipid metabolism or markers of adipocyte differentiation, enzymes involved in detoxification processes, as well as structural components of the cytoskeleton. Some other groups of genes displayed increased expression, such as those encoding inflammatory markers. The results of the microarray analysis were confirmed by semiquantitative RT-PCR studies run on a selected number of genes that were differentially expressed or not modified. |
| Databáze: | OpenAIRE |
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