Photoreversible stretching of a BAPTA chelator marshalling Ca 2+ -binding in aqueous media
| Autor: | Robin Bofinger, Aurélien Ducrot, Arnaud Tron, Nathan D. McClenaghan, Jean-Luc Pozzo, Ingrid Sanz Beguer |
|---|---|
| Přispěvatelé: | Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_element
calcium binding Calcium 010402 general chemistry 01 natural sciences Exocytosis lcsh:QD241-441 chemistry.chemical_compound BAPTA lcsh:Organic chemistry bapta medicine [CHIM]Chemical Sciences lcsh:Science ComputingMilieux_MISCELLANEOUS Photoswitch photoswitch 010405 organic chemistry Chemistry Organic Chemistry 0104 chemical sciences photorelease Dissociation constant azobenzene Azobenzene Second messenger system Biophysics lcsh:Q medicine.symptom Muscle contraction |
| Zdroj: | Beilstein Journal of Organic Chemistry Beilstein Journal of Organic Chemistry, Beilstein-Institut, 2019, 15, pp.2801-2811. ⟨10.3762/bjoc.15.273⟩ Beilstein Journal of Organic Chemistry, Vol 15, Iss 1, Pp 2801-2811 (2019) |
| ISSN: | 1860-5397 |
| DOI: | 10.3762/bjoc.15.273⟩ |
| Popis: | Free calcium ion concentration is known to govern numerous biological processes and indeed calcium acts as an important biological secondary messenger for muscle contraction, neurotransmitter release, ion-channel gating, and exocytosis. As such, the development of molecules with the ability to instantaneously increase or diminish free calcium concentrations potentially allows greater control over certain biological functions. In order to permit remote regulation of Ca2+, a selective BAPTA-type synthetic receptor / host was integrated with a photoswitchable azobenzene motif, which upon photoirradiation would enhance (or diminish) the capacity to bind calcium upon acting on the conformation of the adjacent binding site, rendering it a stronger or weaker binder. Photoswitching was studied in pseudo-physiological conditions (pH 7.2, [KCl] = 100 mM) and dissociation constants for azobenzene cis- and trans-isomers have been determined (0.230 μM and 0.102 μM, respectively). Reversible photoliberation/uptake leading to a variation of free calcium concentration in solution was detected using a fluorescent Ca2+ chemosensor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|