Bacillus cereus biofilm formation on central venous catheters of hospitalised cardiac patients
| Autor: | Antje Hofgaard, Anjum Nasim Sabri, Sarah Finke, Samman Ikram, Yasir Rehman, Adam Heikal, Ole Andreas Økstad |
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| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial 0301 basic medicine 030106 microbiology Bacillus cereus Microbial Sensitivity Tests Aquatic Science Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Drug Resistance Multiple Bacterial Ampicillin medicine Central Venous Catheters Humans Equipment and Supplies Hospital Water Science and Technology Whole genome sequencing biology Biofilm Clindamycin biology.organism_classification Anti-Bacterial Agents Penicillin 030104 developmental biology Cereus Biofilms Catheter-Related Infections Equipment Contamination Bacteria medicine.drug |
| Zdroj: | Biofouling. 35:204-216 |
| ISSN: | 1029-2454 0892-7014 |
| DOI: | 10.1080/08927014.2019.1586889 |
| Popis: | Formation of bacterial biofilms is a risk with many in situ medical devices. Biofilm-forming Bacillus species are associated with potentially life-threatening catheter-related blood stream infections in immunocompromised patients. Here, bacteria were isolated from biofilm-like structures within the lumen of central venous catheters (CVCs) from two patients admitted to cardiac hospital wards. Isolates belonged to the Bacillus cereus group, exhibited strong biofilm formation propensity, and mapped phylogenetically close to the B. cereus emetic cluster. Together, whole genome sequencing and quantitative PCR confirmed that the isolates constituted the same strain and possessed a range of genes important for and up-regulated during biofilm formation. Antimicrobial susceptibility testing demonstrated resistance to trimethoprim-sulphamethoxazole, clindamycin, penicillin and ampicillin. Inspection of the genome revealed several chromosomal beta-lactamase genes and a sulphonamide resistant variant of folP. This study clearly shows that B. cereus persisting in hospital ward environments may constitute a risk factor from repeated contamination of CVCs. |
| Databáze: | OpenAIRE |
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