Further characterisation of the p53 responsive element – identification of new candidate genes for trans-activation by p53
| Autor: | Pierre May, Brigitte Debuire, Valérie Deguin-Chambon, Evelyne May, Jean-Christophe Bourdon, Philippe Dessen, Jean-Claude Lelong |
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| Rok vydání: | 1997 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Transcriptional Activation Cancer Research Candidate gene Sequence analysis Molecular Sequence Data Response element Biology GPI-Linked Proteins Antigens CD Genes Reporter Somatomedins Genetics Consensus sequence Humans Gene family ADP-ribosyl Cyclase Promoter Regions Genetic Molecular Biology Gene Membrane Glycoproteins Base Sequence Promoter Sequence Analysis DNA Regulatory sequence Tumor Suppressor Protein p53 HeLa Cells |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1476-5594 0950-9232 |
| DOI: | 10.1038/sj.onc.1200804 |
| Popis: | The p53 protein is known to trans-activate a number of genes by specific binding to a consensus sequence containing two decamers of the type: PuPuPuCA/TT/AGPyPyPy. In order to identify new p53 trans-activated genes, we defined a set of criteria for computer search of p53-responsive elements. Based on experimental data, we proposed an extended consensus sequence composed of the two decamers of the El-Deiry consensus sequence flanked by two additional ones. A maximum of 3 bp substitutions was accepted for the two decamers of the El-Deiry consensus sequence, as well as for each additional decamer, except when the two decamers of the El-Deiry consensus sequence are contiguous. In this case, each additional decamer is allowed to bear one base insertion or deletion between the median C and G. This set of criteria was validated by identifying within the promoter region of the IGF-BP3 gene the existence of a novel p53-responsive element whose functional significance was verified. By limiting our computer search to Vertebrate genes involved in cell cycle regulation, cellular adhesion or metastatic processes and to gene families most often found in HOVERGEN database, 7785 gene sequences were first analysed. Among the oncogenes, kinases, proteases and structural proteins, 55 new genes were selected; six of them were retrieved in more than one species. |
| Databáze: | OpenAIRE |
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