HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland
| Autor: | Alessandra Estrela-Lima, Geovanni Dantas Cassali, Fernanda Freitas Miranda, André Luís Branco de Barros, Mariana Rezende Alves, Enio Ferreira, Karine Araújo Damasceno, Rafael Malagoli Rocha, Conrado de Oliveira Gamba |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology Receptor ErbB-2 Hibridização in situ Cell Protein Expression lcsh:Medicine Gene Expression 0302 clinical medicine Gene expression Medicine and Health Sciences Dog Diseases RNA Neoplasm lcsh:Science Multidisciplinary biology Invasive Tumors Cell migration Mammary Glands Extracellular Matrix ErbB Receptors Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Versican Immunohistochemistry Female Anatomy Research Article medicine.medical_specialty Histology Mammary Neoplasms Animal In situ hybridization Research and Analysis Methods Carcinomas Tumor misto maligno 03 medical and health sciences Dogs Malignant Tumors Exocrine Glands medicine Gene Expression and Vector Techniques Genetics Animals RNA Messenger Receptores de superfície celular Molecular Biology Techniques Molecular Biology Immunohistochemistry Techniques Metástase neoplásica Molecular Biology Assays and Analysis Techniques lcsh:R Reproductive System Cancers and Neoplasms Biology and Life Sciences Genes ErbB Histochemistry and Cytochemistry Techniques 030104 developmental biology Tumor progression Versicanas biology.protein Cancer research Immunologic Techniques lcsh:Q Neoplasias mamárias animais Breast Tissue Immunostaining |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 8, p e0160419 (2016) Repositório Institucional da UFMG Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
| ISSN: | 1932-6203 |
| Popis: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Versican expression promotes tumor growth by destabilizing focal cell contacts, thus impeding cell adhesion and facilitating cell migration. It not only presents or recruits molecules to the cell surface, but also modulates gene expression levels and coordinates complex signal pathways. Previously, we suggested that the interaction between versican and human epidermal growth factor receptors may be directly associated with tumor aggressiveness. Thus, the expression of EGFR and HER-2 in these neoplasms may contribute to a better understanding of the progression mechanisms in malignant mammary tumors. The purpose of this study was to correlate the gene and protein expressions of EGFR and HER2 by RNA In Situ Hybridization (ISH) and immunohistochemistry (IHC), respectively, and their relationship with the versican expression in carcinomas in mixed tumors and carcinosarcomas of the canine mammary gland. The results revealed that EGFR mRNA expression showed a significant difference between in situ and invasive carcinomatous areas in low and high versican expression groups. Identical results were observed in HER-2 mRNA expression. In immunohistochemistry analysis, neoplasms with low versican expression. showed greater EGFR immunostaining in the in situ areas than in invasive areas, even as the group presenting high versican expression displayed greater EGFR and HER-2 staining in in situ areas. Significant EGFR and HER-2 mRNA and protein expressions in in situ carcinomatous sites relative to invasive areas suggest that these molecules play a role during the early stages of tumor progression. |
| Databáze: | OpenAIRE |
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