In-Depth Investigation of Archival and Prospectively Collected Samples Reveals No Evidence for XMRV Infection in Prostate Cancer
Autor: | Graham Simmons, Anatoly Urisman, Gerald Schochetman, Ning Tang, Imke Steffen, Nicole Fischer, John Hackett, Robert H. Silverman, Jaydip Das Gupta, Deanna Lee, Eric A. Klein, Christina Gaughan, Xiaoxing Qiu, Ross J. Molinaro, Joseph L. DeRisi, Charles Y. Chiu, Ka Cheung Luk, Miranda Broz, Don Ganem |
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Rok vydání: | 2012 |
Předmět: |
RNA viruses
Male Research Validity Databases Factual Microarrays Xenotropic murine leukemia virus-related virus lcsh:Medicine Gene Expression urologic and male genital diseases Cohort Studies Mice Prostate cancer Viral classification Prostate Emerging Viral Diseases Zoonoses Molecular Cell Biology Basic Cancer Research Murine leukemia virus Pathology Genome Sequencing Prospective Studies lcsh:Science Gammaretrovirus 0303 health sciences Multidisciplinary Zoonotic Diseases Cancer Risk Factors Prostate Cancer Prostate Diseases virus diseases Genomics Research Assessment Clinical Laboratory Sciences Mitochondria 3. Good health medicine.anatomical_structure Oncology Veterinary Diseases Cytogenetic Analysis Medicine Infectious diseases RNA extraction Research Laboratories Sequence Analysis Molecular Pathology Research Article Clinical Pathology Science Policy Urology Retrovirology and HIV immunopathogenesis Viral and Bacterial Causes of Cancer Viral diseases Genome Viral Biology Genome Complexity Microbiology Polymorphism Single Nucleotide Virus Specimen Handling Molecular Genetics Cytogenetics 03 medical and health sciences Diagnostic Medicine Virology Cell Line Tumor Retroviruses Genetics medicine Chronic fatigue syndrome Animals Humans Microbial Pathogens Research Errors 030304 developmental biology Prostatectomy 030306 microbiology lcsh:R Computational Biology HIV Cancers and Neoplasms Prostatic Neoplasms medicine.disease biology.organism_classification Viral Disease Diagnosis Genitourinary Tract Tumors Viruses and Cancer RNA lcsh:Q Veterinary Science Metagenomics Viral Transmission and Infection General Pathology |
Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 9, p e44954 (2012) |
ISSN: | 1932-6203 |
Popis: | XMRV, or xenotropic murine leukemia virus (MLV)-related virus, is a novel gammaretrovirus originally identified in studies that analyzed tissue from prostate cancer patients in 2006 and blood from patients with chronic fatigue syndrome (CFS) in 2009. However, a large number of subsequent studies failed to confirm a link between XMRV infection and CFS or prostate cancer. On the contrary, recent evidence indicates that XMRV is a contaminant originating from the recombination of two mouse endogenous retroviruses during passaging of a prostate tumor xenograft (CWR22) in mice, generating laboratory-derived cell lines that are XMRV-infected. To confirm or refute an association between XMRV and prostate cancer, we analyzed prostate cancer tissues and plasma from a prospectively collected cohort of 39 patients as well as archival RNA and prostate tissue from the original 2006 study. Despite comprehensive microarray, PCR, FISH, and serological testing, XMRV was not detected in any of the newly collected samples or in archival tissue, although archival RNA remained XMRV-positive. Notably, archival VP62 prostate tissue, from which the prototype XMRV strain was derived, tested negative for XMRV on re-analysis. Analysis of viral genomic and human mitochondrial sequences revealed that all previously characterized XMRV strains are identical and that the archival RNA had been contaminated by an XMRV-infected laboratory cell line. These findings reveal no association between XMRV and prostate cancer, and underscore the conclusion that XMRV is not a naturally acquired human infection. |
Databáze: | OpenAIRE |
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