In-Depth Investigation of Archival and Prospectively Collected Samples Reveals No Evidence for XMRV Infection in Prostate Cancer

Autor: Graham Simmons, Anatoly Urisman, Gerald Schochetman, Ning Tang, Imke Steffen, Nicole Fischer, John Hackett, Robert H. Silverman, Jaydip Das Gupta, Deanna Lee, Eric A. Klein, Christina Gaughan, Xiaoxing Qiu, Ross J. Molinaro, Joseph L. DeRisi, Charles Y. Chiu, Ka Cheung Luk, Miranda Broz, Don Ganem
Rok vydání: 2012
Předmět:
RNA viruses
Male
Research Validity
Databases
Factual
Microarrays
Xenotropic murine leukemia virus-related virus
lcsh:Medicine
Gene Expression
urologic and male genital diseases
Cohort Studies
Mice
Prostate cancer
Viral classification
Prostate
Emerging Viral Diseases
Zoonoses
Molecular Cell Biology
Basic Cancer Research
Murine leukemia virus
Pathology
Genome Sequencing
Prospective Studies
lcsh:Science
Gammaretrovirus
0303 health sciences
Multidisciplinary
Zoonotic Diseases
Cancer Risk Factors
Prostate Cancer
Prostate Diseases
virus diseases
Genomics
Research Assessment
Clinical Laboratory Sciences
Mitochondria
3. Good health
medicine.anatomical_structure
Oncology
Veterinary Diseases
Cytogenetic Analysis
Medicine
Infectious diseases
RNA extraction
Research Laboratories
Sequence Analysis
Molecular Pathology
Research Article
Clinical Pathology
Science Policy
Urology
Retrovirology and HIV immunopathogenesis
Viral and Bacterial Causes of Cancer
Viral diseases
Genome
Viral
Biology
Genome Complexity
Microbiology
Polymorphism
Single Nucleotide
Virus
Specimen Handling
Molecular Genetics
Cytogenetics
03 medical and health sciences
Diagnostic Medicine
Virology
Cell Line
Tumor
Retroviruses
Genetics
medicine
Chronic fatigue syndrome
Animals
Humans
Microbial Pathogens
Research Errors
030304 developmental biology
Prostatectomy
030306 microbiology
lcsh:R
Computational Biology
HIV
Cancers and Neoplasms
Prostatic Neoplasms
medicine.disease
biology.organism_classification
Viral Disease Diagnosis
Genitourinary Tract Tumors
Viruses and Cancer
RNA
lcsh:Q
Veterinary Science
Metagenomics
Viral Transmission and Infection
General Pathology
Zdroj: PLoS ONE
PLoS ONE, Vol 7, Iss 9, p e44954 (2012)
ISSN: 1932-6203
Popis: XMRV, or xenotropic murine leukemia virus (MLV)-related virus, is a novel gammaretrovirus originally identified in studies that analyzed tissue from prostate cancer patients in 2006 and blood from patients with chronic fatigue syndrome (CFS) in 2009. However, a large number of subsequent studies failed to confirm a link between XMRV infection and CFS or prostate cancer. On the contrary, recent evidence indicates that XMRV is a contaminant originating from the recombination of two mouse endogenous retroviruses during passaging of a prostate tumor xenograft (CWR22) in mice, generating laboratory-derived cell lines that are XMRV-infected. To confirm or refute an association between XMRV and prostate cancer, we analyzed prostate cancer tissues and plasma from a prospectively collected cohort of 39 patients as well as archival RNA and prostate tissue from the original 2006 study. Despite comprehensive microarray, PCR, FISH, and serological testing, XMRV was not detected in any of the newly collected samples or in archival tissue, although archival RNA remained XMRV-positive. Notably, archival VP62 prostate tissue, from which the prototype XMRV strain was derived, tested negative for XMRV on re-analysis. Analysis of viral genomic and human mitochondrial sequences revealed that all previously characterized XMRV strains are identical and that the archival RNA had been contaminated by an XMRV-infected laboratory cell line. These findings reveal no association between XMRV and prostate cancer, and underscore the conclusion that XMRV is not a naturally acquired human infection.
Databáze: OpenAIRE
Externí odkaz: