Nutraceutical Characterization of Anthocyanin-Rich Fruits Produced by 'Sun Black' Tomato Line
| Autor: | Giovanni Mita, Andrea Mazzucato, Isabella Nicoletti, Helge Berland, Maurizio Enea Picarella, Carmela Gerardi, Miriana Durante, Øyvind M. Andersen, Gabriele Maiorano, Federica Blando |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism lcsh:TX341-641 030209 endocrinology & metabolism tomato functional food 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Chlorogenic acid Petunidin Wild tomato Food science Carotenoid functional foods Nutrition Original Research chemistry.chemical_classification bioactive compounds 030109 nutrition & dietetics Nutrition and Dietetics tomato breeding biology Phenolic acid biology.organism_classification anthocyanins Malvidin chemistry Anthocyanin Solanum lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Frontiers in Nutrition Frontiers in Nutrition, Vol 6 (2019) Frontiers in nutrition Online 6 (2019). doi:10.3389/fnut.2019.00133 info:cnr-pdr/source/autori:Blando F, Berland H, Maiorano G, Durante M, Mazzucato A, Picarella ME, Nicoletti I, Gerardi C, Mita G, Andersen OM/titolo:Nutraceutical characterization of anthocyanin-rich fruits produced by 'Sun Black' tomato lines/doi:10.3389%2Ffnut.2019.00133/rivista:Frontiers in nutrition Online/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:6 |
| ISSN: | 2296-861X |
| DOI: | 10.3389/fnut.2019.00133 |
| Popis: | Tomato (Solanum lycopersicum L.) is one of the most cultivated vegetable in the world and it represents a large source of bioactive compounds, including carotenoids and polyphenols (phenolic acids and flavonoids). However, the concentration of flavonoids in tomato is considered sub-optimal, particularly because anthocyanins are not generally present. Therefore, this crop has been the object of an intense metabolic engineering in order to obtain anthocyanin-enriched tomatoes by using either breeding or transgenic strategies. Some wild tomato species, such as S. chilense and S. cheesmaniae, biosynthesize anthocyanins in the fruit sub-epidermal tissue, and some alleles from those genotypes have been introgressed into a new developed purple tomato line, called “Sun Black” (SB). It is a tomato line with a purple skin color, both in green and in red fruit stages, due to the biosynthesis of anthocyanins in the peel, and a normal red color pulp, with a taste just like a traditional tomato. SB is the result of a breeding programme and it is not a genetically modified (GM) product. We report the chemical characterization and structure elucidation of the attractive anthocyanins found in the peel of SB tomato, as well as other bioactive compounds (carotenoids, polyphenols, vitamin C) of the whole fruit. Using one- and two-dimensional NMR experiments, the two main anthocyanins were identified to be petunidin 3-O-[6″-O-(4‴-O-E-p-coumaroyl-α-rhamnopyranosyl) -β-glucopyranoside]-5-O-β-glucopyranoside (petanin) and malvidin 3-O-[6″-O-(4‴-O-E-p-coumaroyl-α-rhamnopyranosyl)-β-glucopyranoside]-5-O-β-glucopyranoside (negretein). The total anthocyanins in the whole ripe fruit was 1.2 mg/g dry weight (DW); 7.1 mg/100 g fresh weight (FW). Chlorogenic acid (the most abundant phenolic acid) was 0.6 mg/g DW; 3.7 mg/100 g FW. The main flavonol, rutin was 0.8 mg/g DW; 5 mg/100 g FW. The total carotenoid content was 211.3 μg/g DW; 1,268 μg/100 g FW. The total phenolic content was 8.6 mg/g DW; 52.2 mg/100 g FW. The vitamin C content was 37.3 mg/100 g FW. The antioxidant activities as measured by the TEAC and ORAC assays were 31.6 and 140.3 μmol TE/g DW, respectively (193 and 855.8 μmol TE/100 g FW, respectively). The results show the unique features of this new tomato genotype with nutraceutical properties. publishedVersion |
| Databáze: | OpenAIRE |
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