Characterization of a porcine variable number tandem repeat sequence specific for the glucosephosphate isomerase locus
| Autor: | I. Harbitz, S. Kran, Tom Kristensen, W. Davies |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Genetics
Base Sequence Swine Molecular Sequence Data Glucose-6-Phosphate Isomerase Intron Nucleic acid sequence Locus (genetics) General Medicine DNA Satellite Biology Molecular biology Variable number tandem repeat genomic DNA Subcloning STR analysis Tandem repeat Animals Animal Science and Zoology Cloning Molecular Repetitive Sequences Nucleic Acid |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1365-2052 0268-9146 |
| DOI: | 10.1111/j.1365-2052.1992.tb02163.x |
| Popis: | Summary. A variable number of tandem repeat from a porcine glucosephosphate isomerase intron has been isolated and sequenced. The repeat has a unit size of 39 bp, is highly conserved and is present in at least 14 copies. Flanking sequences show a sequence periodicity of 53-54bp and some sequence homology to the 39 bp repeat. A considerable part of the genomic DNA has been lost during subcloning and is considered to be deletion prone or refractory to propagation in E. coli. The tandem repeat is locus specific and detects at least six alleles in Bam HI digested porcine DNA. No homology to other tandem repeat sequences has been found. |
| Databáze: | OpenAIRE |
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