Transcriptional profiling of the spleen in progressive visceral leishmaniasis reveals mixed expression of type 1 and type 2 cytokine-responsive genes
| Autor: | Alvaro G. Hernandez, Peter C. Melby, A. Medina, Mandakini Patel, Omar A. Saldarriaga, Bruno L. Travi, Michael Cappello, Claudia M. Espitia, E. Yaneth Osorio, Mark Band, Andrew Pekosz |
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| Jazyk: | angličtina |
| Předmět: |
Transcriptional profiling
endocrine system DNA Complementary animal diseases Immunology Leishmania donovani Hamster Microarray Interferon-gamma Cricetinae parasitic diseases medicine Animals Cluster Analysis Humans RNA Messenger Oligonucleotide Array Sequence Analysis Expressed Sequence Tags Visceral leishmaniasis Regulation of gene expression Principal Component Analysis Mesocricetus biology Gene Expression Profiling Molecular Sequence Annotation Leishmaniasis medicine.disease biology.organism_classification Up-Regulation Gene expression profiling Gene Ontology Gene Expression Regulation Disease Progression Cytokines Leishmaniasis Visceral Interleukin-4 Spleen Signal Transduction Research Article Golden hamster |
| Zdroj: | BMC Immunology |
| ISSN: | 1471-2172 |
| DOI: | 10.1186/s12865-014-0038-z |
| Popis: | Background The Syrian golden hamster (Mesocricetus aureus) has been used as a model to study infections caused by a number of human pathogens. Studies of immunopathogenesis in hamster infection models are challenging because of the limited availability of reagents needed to define cellular and molecular determinants. Results We sequenced a hamster cDNA library and developed a first-generation custom cDNA microarray that included 5131 unique cDNAs enriched for immune response genes. We used this microarray to interrogate the hamster spleen response to Leishmania donovani, an intracellular protozoan that causes visceral leishmaniasis. The hamster model of visceral leishmaniasis is of particular interest because it recapitulates clinical and immunopathological features of human disease, including cachexia, massive splenomegaly, pancytopenia, immunosuppression, and ultimately death. In the microarray a differentially expressed transcript was identified as having at least a 2-fold change in expression between uninfected and infected groups and a False Discovery Rate of |
| Databáze: | OpenAIRE |
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