Phenotypic traits of Burkholderia spp. associated with ecological adaptation and plant-host interaction
| Autor: | Emy T. Mano, Miguel A. Valvano, Manuella Nóbrega Dourado, Leandro M. Garrido, Karent J. Romero-Gutiérrez, Luiz Ricardo Olchanheski, Francisco Dini-Andreote, Welington Luiz Araújo |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Genomic Islands
Genotype Burkholderia Acclimatization Adaptation Biological Microbiology 03 medical and health sciences Anti-Infective Agents RNA Ribosomal 16S Genetic variation Endophytes Orchidaceae Symbiosis Phylogeny Plant Diseases 030304 developmental biology 0303 health sciences Rhizosphere Host Microbial Interactions biology 030306 microbiology Ecology Host (biology) Burkholderia Infections Drug Resistance Microbial Phenotypic trait biology.organism_classification Oxidative Stress BACTÉRIAS PATOGÊNICAS Phenotype Biological Control Agents Genes Bacterial Lipid Peroxidation Adaptation Transcriptome Niche adaptation Bacteria Multilocus Sequence Typing |
| Zdroj: | Romero-Gutiérrez, K J, Dourado, M N, Garrido, L M, Olchanheski, L R, Mano, E T, Dini-Andreote, F, Valvano, M A & Araújo, W L 2020, ' Phenotypic traits of Burkholderia spp. associated with ecological adaptation and plant-host interaction ', Microbiological Research . https://doi.org/10.1016/j.micres.2020.126451 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Burkholderia species have different lifestyles establishing mutualist or pathogenic associations with plants and animals. Changes in the ecological behavior of these bacteria may depend on genetic variations in response to niche adaptation. Here, we studied 15 Burkholderia strains isolated from different environments with respect to genetic and phenotypic traits. By Multilocus Sequence Analysis (MLSA) these isolates fell into 6 distinct groups. MLSA clusters did not correlate with strain antibiotic sensitivity, but with the bacterial ability to produce antimicrobial compounds and control orchid necrosis. Further, the B. seminalis strain TC3.4.2R3, a mutualistic bacterium, was inoculated into orchid plants and the interaction with the host was evaluated by analyzing the plant response and the bacterial oxidative stress response in planta. TC3.4.2R3 responded to plant colonization by increasing its own growth rate and by differential gene regulation upon oxidative stress caused by the plant, while reducing the plant's membrane lipid peroxidation. The bacterial responses to oxidative stress were recapitulated by bacterial exposure to the herbicide paraquat. We suggest that the ability of Burkholderia species to successfully establish in the rhizosphere correlates with genetic variation, whereas traits associated with antibiotic resistance are more likely to be categorized as strain specific. |
| Databáze: | OpenAIRE |
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