Augmentation by aphidicolin of 1-β-d-arabinofuranosylcytosine-induced c-jun and NF-κB activation in a human myeloid leukemia cell line: Correlation with apoptosis
| Autor: | Ying Wei Lin, Akihiro Kataoka, Akiro Okuda, Katsuji Kuwakado, Ikuya Usami, Rikimaru Bessho, Yoshihiro Wakazono, Masaru Kubota |
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| Rok vydání: | 1995 |
| Předmět: |
Aphidicolin
Antimetabolites Antineoplastic Cancer Research Programmed cell death Proto-Oncogene Proteins c-jun Molecular Sequence Data Genes myc Apoptosis Biology chemistry.chemical_compound Genes jun Tumor Cells Cultured medicine Humans Electrophoretic mobility shift assay RNA Messenger Enzyme Inhibitors Base Sequence c-jun Cytarabine NF-kappa B food and beverages Myeloid leukemia Drug Synergism DNA Polymerase II Hematology biochemical phenomena metabolism and nutrition Molecular biology Gene Expression Regulation Neoplastic carbohydrates (lipids) Oligodeoxyribonucleotides Oncology chemistry Cell culture DNA Damage medicine.drug |
| Zdroj: | Leukemia Research. 19:645-650 |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/0145-2126(95)00046-q |
| Popis: | 1-beta-D-arabinofuranosylcytosine (ara-C) (2 microM) can induce apoptosis in a human myeloid leukemia cell line, U937, after 4 h of incubation. Pretreatment of cells with aphidicolin (2 microM) augments ara-C-induced apoptosis, since it was first observed at 0.4 microM ara-C and became more intense at 2 and 10 microM. Although aphidicolin itself had a marginal effect on c-jun expression, it significantly augmented ara-C induced c-jun upregulation by shortening the lag time and lowering ara-C concentrations necessary for the induction of detectable c-jun transcripts. Aphidicolin and ara-C acted synergistically to increase NF-kappa B DNA binding activity as determined by an electrophoretic mobility shift assay. Expression of c-myc was slightly increased through the DNA degradative phase, and was then downregulated. Thus, the activation of NF-kappa B and c-jun expression seems to be well correlated with the potentiation by aphidicolin of ara-C-induced apoptosis. |
| Databáze: | OpenAIRE |
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