Photodynamic and Antibiotic Therapy Impair the Pathogenesis of Enterococcus faecium in a Whole Animal Insect Model
| Autor: | Louis B. Rice, José Chibebe Junior, Michael R. Hamblin, Antonio Olavo Cardoso Jorge, George P. Tegos, Michael S. Gilmore, Martha S. Ribeiro, Juliana Campos Junqueira, Eleftherios Mylonakis, Caetano P. Sabino, Beth Burgwyn Fuchs |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Massachusetts General Hospital, Faculty of Pindamonhangaba, Nuclear and Energy Research Institute, Harvard Medical School, Brown University, University of New Mexico, Massachusetts Institute of Technology (MIT), Harvard University--MIT Division of Health Sciences and Technology, Hamblin, Michael R. |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
antibiotic resistance
vancomycin Antibiotics Enterococcus faecium drug response lcsh:Medicine Pathogenesis Moths medicine.disease_cause antibiotic therapy lcsh:Science 0303 health sciences combination chemotherapy Multidisciplinary Photosensitizing Agents biology pathogenesis Combination chemotherapy Animal Models Antimicrobial infection control unclassified drug 3. Good health Bacterial Pathogens Anti-Bacterial Agents Galleria mellonella Host-Pathogen Interaction Infectious Diseases photodynamic therapy Vancomycin Medicine medicine.drug Research Article survival rate streptomycin Infectious Disease Control vancomycin resistant Enterococcus medicine.drug_class Clinical Research Design photosensitizing agent gentamicin Microbiology enterococcal infection Enterococcus faecalis in vivo study 03 medical and health sciences Model Organisms Virology Microbial Control medicine Animals controlled study Vancomycin-resistant Enterococcus Animal Models of Disease phenothiazinium Biology Gram-Positive Bacterial Infections 030304 developmental biology Gram Positive nonhuman 030306 microbiology lcsh:R fungi biochemical phenomena metabolism and nutrition bacterial strain biology.organism_classification antibiotic sensitivity drug efficacy Methylene Blue Animal Models of Infection Disease Models Animal Photochemotherapy ampicillin caterpillar lcsh:Q Zoology Entomology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 2, p e55926 (2013) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP PLoS |
| ISSN: | 1932-6203 |
| Popis: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:28:27Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:48:09Z : No. of bitstreams: 1 2-s2.0-84874006080.pdf: 650584 bytes, checksum: 7c5b49e68362d89881ef802f5396e712 (MD5) Made available in DSpace on 2014-05-27T11:28:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-02-14 Enterococcus faecium has emerged as one of the most important pathogens in healthcare-associated infections worldwide due to its intrinsic and acquired resistance to many antibiotics, including vancomycin. Antimicrobial photodynamic therapy (aPDT) is an alternative therapeutic platform that is currently under investigation for the control and treatment of infections. PDT is based on the use of photoactive dye molecules, widely known as photosensitizer (PS). PS, upon irradiation with visible light, produces reactive oxygen species that can destroy lipids and proteins causing cell death. We employed Galleria mellonella (the greater wax moth) caterpillar fatally infected with E. faecium to develop an invertebrate host model system that can be used to study the antimicrobial PDT (alone or combined with antibiotics). In the establishment of infection by E. faecium in G. mellonella, we found that the G. mellonella death rate was dependent on the number of bacterial cells injected into the insect hemocoel and all E. faecium strains tested were capable of infecting and killing G. mellonella. Antibiotic treatment with ampicillin, gentamicin or the combination of ampicillin and gentamicin prolonged caterpillar survival infected by E. faecium (P = 0.0003, P = 0.0001 and P = 0.0001, respectively). In the study of antimicrobial PDT, we verified that methylene blue (MB) injected into the insect followed by whole body illumination prolonged the caterpillar survival (P = 0.0192). Interestingly, combination therapy of larvae infected with vancomycin-resistant E. faecium, with antimicrobial PDT followed by vancomycin, significantly prolonged the survival of the caterpillars when compared to either antimicrobial PDT (P = 0.0095) or vancomycin treatment alone (P = 0.0025), suggesting that the aPDT made the vancomycin resistant E. faecium strain more susceptible to vancomycin action. In summary, G. mellonella provides an invertebrate model host to study the antimicrobial PDT and to explore combinatorial aPDT-based treatments. Department of Biosciences and Oral Diagnosis Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo Division of Infectious Diseases Massachusetts General Hospital, Boston, MA Department of Restorative Dentistry Faculty of Pindamonhangaba, Pindamonhangaba, São Paulo Wellman Center for Photomedicine Massachusetts General Hospital, Boston, MA Center for Lasers and Applications Nuclear and Energy Research Institute, São Paulo, São Paulo Massachusetts Eye and Ear Infirmary Harvard Medical School, Boston, MA Warren Alpert Medical School Brown University/Rhode Island and Miriam Hospitals, Providence, RI Department of Dermatology Harvard Medical School, Boston, MA Department of Pathology and Center for Molecular Discovery University of New Mexico, Albuquerque, NM Division of Health Sciences and Technology Harvard-Massachusetts Institute of Technology, Cambridge, MA Department of Biosciences and Oral Diagnosis Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo |
| Databáze: | OpenAIRE |
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