Assembly and release of HIV-1 precursor Pr55gag virus-like particles from recombinant baculovirus-infected insect cells
| Autor: | Denise Thines, Clotilde Thiriart, Myriam Francotte, Eric Jacobs, Françoise de Foresta, Michel De Wilde, D. Gheysen |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Cell
Gene Products gag Insect Viruses Biology Virus Replication General Biochemistry Genetics and Molecular Biology Virus law.invention Viral life cycle law medicine Animals Protein Precursors Myristoylation Budding Myristates Virion Proteins Genes gag Molecular biology Recombinant Proteins Cell biology Lepidoptera medicine.anatomical_structure Cytoplasm Protein Biosynthesis HIV-1 Recombinant DNA Heterologous expression |
| Zdroj: | Cell. 59:103-112 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(89)90873-8 |
| Popis: | The unprocessed Gag precursor from HIV-1, when expressed in recombinant baculovirus-infected insect cells, is targeted to the plasma membrane and assembles in 100–120 nm particles budding from the cell surface. This process mimics HIV immature particle formation and is dependent on myristoylation of the N-terminal glycine, as deletion of the latter results in particle accumulation in the cytoplasm and, interestingly, in the nucleus, pointing to a potential role of this non-fatty-acid-acylated species in the viral life cycle. Inclusion of the pol gene in the construct results in efficient processing of Pr55 gag and a pronounced decrease in particle formation. Deletion of the C terminus (p16) of the Gag precursor, including the finger domains, abolishes particle assembly, but membrane targeting and evagination still occur. Heterologous expression in insect cells may prove very useful for the study of the molecular events leading to retroviral particle morphogenesis. |
| Databáze: | OpenAIRE |
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