Requirement of regulated endocrine-specific protein-18 for development and expression of regulated endocrine-specific protein-18 isoform c in mice
| Autor: | Jian Fei, Yufang Huang, Jie Liu, Jian Ling Yang, Shu Ping Gu, Yan Cong Wang, Min Juan Bian, Mei Yu, Fang Huang, Min Liang, Xiao Qi Hong |
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| Rok vydání: | 2010 |
| Předmět: |
Gene isoform
Molecular Sequence Data Embryonic Development Endocrine System Nerve Tissue Proteins Biology Mice In vivo Genetics Endocrine system Animals Protein Isoforms Cloning Molecular Molecular Biology DNA Primers Cloning Messenger RNA Base Sequence Reverse Transcriptase Polymerase Chain Reaction Dopaminergic Gene targeting Embryo General Medicine Sequence Analysis DNA Embryo Mammalian Molecular biology Mice Mutant Strains Gene Targeting |
| Zdroj: | Molecular biology reports. 38(4) |
| ISSN: | 1573-4978 |
| Popis: | Regulated endocrine-specific protein-18 (RESP18) is distributed mainly in the peripheral endocrine and neuroendocrine tissues. The expression of RESP18 protein is regulated by physiological factors, such as blood glucose or dopaminergic drugs, but its functions remain unclear. In this study, to explore the biological functions of RESP18 in vivo, we generated RESP18 heterozygous deficient mice, and further found RESP18 was essential for embryonic development. In addition, we cloned a new isoform of mouse RESP18 by reverse transcription-polymerase chain reaction (RT-PCR), and denominated it as RESP18-c. Mouse RESP18-c, by skipping exon4 (43 bp in length), encodes a shorter protein of 120 amino acid residues. The distribution of RESP18-c mRNA is similar with that of RESP18 mRNA in the peripheral tissues and brains of mice. |
| Databáze: | OpenAIRE |
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