The structure of DNA-DLPC-cationic gemini surfactant aggregates: a small angle synchrotron X-ray diffraction study
| Autor: | Mária Hanulová, Ivan Lacko, Ferdinand Devínsky, Sérgio S. Funari, Daniela Uhríková, Pavol Balgavý |
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| Rok vydání: | 2004 |
| Předmět: |
Lipid Bilayers
Biophysics chemistry [Lipid Bilayers] Butylamines Biochemistry butane-1 4-diylbis(dodecyldimethylammonium bromide) law.invention chemistry.chemical_compound Surface-Active Agents Lamellar phase Pulmonary surfactant X-Ray Diffraction law Bromide Phase (matter) chemistry [Phosphatidylcholines] Lipid bilayer chemistry [Surface-Active Agents] chemistry [DNA] Models Statistical Vesicle Organic Chemistry 1 2-dilauroylphosphatidylcholine Temperature DNA chemistry [Butylamines] Synchrotron chemistry [Quaternary Ammonium Compounds] Quaternary Ammonium Compounds Crystallography chemistry Models Chemical ddc:540 X-ray crystallography Phosphatidylcholines Thermodynamics Synchrotrons |
| Zdroj: | Biophysical chemistry 111, 197-204 (2004). doi:10.1016/j.bpc.2004.05.012 |
| ISSN: | 0301-4622 |
| DOI: | 10.1016/j.bpc.2004.05.012 |
| Popis: | The structure of aggregates formed by interaction of DNA with unilamellar dilauroylphosphatidylcholine (DLPC) vesicles (DNA:DLPC=1:1 base/mol) in the presence of gemini surfactant butane-1,4-diyl-bis(dodecyldimethylammonium bromide) (C12GS) was investigated using synchrotron small angle X-ray diffraction. In the concentration range C12GS+:DLPC< or =1 mol/mol, a condensed lamellar Lalphac phase was found with a repeat period of lipid bilayer stacking in the range d approximately 5.70-6.53 nm and the DNA interhelical distance d(DNA) approximately 3.52-3.99 nm, depending on the concentration of C12GS. At molar ratio C12GS+:DLPC> or =0.35:1, the diffractograms have shown the presence of a second lamellar phase with the repeat period d approximately 5.31 nm which slightly decreases with increasing concentration of C12GS+. The increasing fraction of this phase in the aggregates with increasing concentration of C12GS supports the association of this phase with microscopic domains enriched by surfactant molecules. The temperature behaviour of aggregates was investigated in the range 25-60 degrees C and the transversal thermal expansivities of the observed phases were determined. |
| Databáze: | OpenAIRE |
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