Analysis of HLA-DRB1*0901-binding HPV-16 E7 helper T cell epitope+
| Autor: | Mitsuo Okubo, Masami Yanagisawa, Maki Saito, Hiroo Maeda, Katsuyuki Kinoshita, Hiroki Inoku, Ranko Hirata, Satoru Takeda |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
CD4-Positive T-Lymphocytes
Papillomavirus E7 Proteins T cell medicine.medical_treatment Molecular Sequence Data Uterine Cervical Neoplasms Human leukocyte antigen Epitope Epitopes medicine HLA-DR Humans Amino Acid Sequence Papillomaviridae HLA-DRB1 Cells Cultured Antigen Presentation biology Obstetrics and Gynecology HLA-DR Antigens Oncogene Proteins Viral T-Lymphocytes Helper-Inducer Immunotherapy Uterine Cervical Dysplasia biology.organism_classification Interleukin-12 Molecular biology medicine.anatomical_structure DNA Viral Female HLA-DRB1 Chains |
| Zdroj: | Journal of Obstetrics and Gynaecology Research. 30:120-129 |
| ISSN: | 1447-0756 1341-8076 |
| DOI: | 10.1111/j.1447-0756.2003.00171.x |
| Popis: | Aim: This study sought to determine the human papillomavirus (HPV)-16 E7 epitopes that would be presented by HLA-DR molecules to CD4-positive T cells in patients with cervical carcinoma. Methods: HLA-DR binding assays were performed using HPV-16 E7-derived synthetic peptides and, after incubation with these DR-binding peptides, helper T cell frequencies were analyzed in patients whose HLA and HPV genotypes were confirmed. Results: We determined that the E7d peptide, 61CDSTLRLCVQSTHVDIRTL80E, was bound by HLA-DRB1*0901. An increased frequency (0.3–2.4%) of type 2 helper T cell responses was found in HLA-DRB1*0901-positive patients with cervical dysplasia and carcinoma. We found that when IL-12 was combined with E7d-peptide stimulation in vitro, the frequency of type 1 helper T cell responses also increased in patients with carcinoma. Conclusion: Thus HPV-16 E7d peptide as an HLA-DRB1*0901-restricted helper T cell epitope might usefully be incorporated into an understanding of the immunological mechanism and immunotherapy for this disease. |
| Databáze: | OpenAIRE |
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