Validation and Application of a Derivatization-Free RP-HPLC-DAD Method for the Determination of Low Molecular Weight Salivary Metabolites
| Autor: | Edoardo Benedetti, Riccardo Nieri, Emilia Bramanti, Beatrice Campanella, Massimo Onor, Tommaso Lomonaco |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
carboxylic acids
Saliva Health Toxicology and Mutagenesis HPLC-DAD multianalyte analysis lcsh:Medicine 01 natural sciences Article Fluorescence 03 medical and health sciences chemistry.chemical_compound fluids and secretions stomatognathic system organic acids Humans Derivatization saliva amino carboxylic acid hydroxy organic acids 030304 developmental biology Sample handling chemistry.chemical_classification Chromatography Reverse-Phase 0303 health sciences Chromatography Molecular Structure Chemistry Biomolecule fungi 010401 analytical chemistry lcsh:R Public Health Environmental and Occupational Health Rp hplc dad food and beverages Reproducibility of Results Diode array 0104 chemical sciences Molecular Weight Potential biomarkers After treatment |
| Zdroj: | International Journal of Environmental Research and Public Health, Vol 17, Iss 6158, p 6158 (2020) International journal of environmental research and public health (Online) 17 (2020). doi:10.3390/ijerph17176158 info:cnr-pdr/source/autori:Campanella B.; Lomonaco T.; Benedetti E.; Onor M.; Nieri R.; Bramanti E./titolo:Validation and Application of a Derivatization-Free RP-HPLC-DAD Method for the Determination of Low Molecular Weight Salivary Metabolites/doi:10.3390%2Fijerph17176158/rivista:International journal of environmental research and public health (Online)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:17 International Journal of Environmental Research and Public Health Volume 17 Issue 17 |
| ISSN: | 1661-7827 1660-4601 |
| Popis: | Saliva is an interesting, non-conventional, valuable diagnostic fluid. It can be collected using standardized sampling device thus, its sampling is easy and non-invasive, it contains a variety of organic metabolites that reflect blood composition. The aim of this study was to validate a user-friendly method for the simultaneous determination of low molecular weight metabolites in saliva. We have optimized and validated a high throughput, direct, low-cost reversed phase liquid chromatographic method with diode array detection method without any pre- or post-column derivatization. We indexed salivary biomolecules in 35 whole non-stimulated saliva samples collected in 8 individuals in different days, including organic acids and amino acids and other carbonyl compounds. Among these, 16 whole saliva samples were collected by a single individual over three weeks before, during and after treatment with antibiotic in order to investigate the dynamics of metabolites. The concentrations of the metabolites were compared with the literature data. The multianalyte method here proposed requires a minimal sample handling and it is cost-effectiveness as it makes possible to analyze a high number of samples with basic instrumentation. The identification and quantitation of salivary metabolites may allow the definition of potential biomarkers for non-invasive &ldquo personal monitoring&rdquo during drug treatments, work out, or life habits over time. |
| Databáze: | OpenAIRE |
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