Focal accumulation of preribosomes outside the nucleolus during metaphase-anaphase in budding yeast
| Autor: | Blanca Nieto, Giulia Moriggi, Xosé R. Bustelo, Sonia G. Gaspar, Mercedes Dosil |
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| Přispěvatelé: | Worldwide Cancer Research, Ministerio de Economía y Competitividad (España), Fundación Ramón Areces, Universidad de Salamanca, Junta de Castilla y León, European Commission, Banco Santander |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nucleolus Preribosome Saccharomyces cerevisiae Intracellular Space Gene Expression Ribosome DNA Ribosomal Article 03 medical and health sciences Nucleolar functions Genes Reporter Ribosome synthesis Eukaryotic Small Ribosomal Subunit Molecular Biology Ribosomal DNA Mitosis Metaphase Anaphase biology Biological Transport Cell Cycle Checkpoints biology.organism_classification Cell biology Preribosomes 030104 developmental biology RNA Ribosomal Saccharomycetales Ribosomes Cell Nucleolus |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1469-9001 1355-8382 |
| Popis: | Saccharomyces cerevisiae contains one nucleolus that remains intact in the mother-cell side of the nucleus throughout most of mitosis. Based on this, it is assumed that the bulk of ribosome production during cell division occurs in the mother cell. Here, we show that the ribosome synthesis machinery localizes not only in the nucleolus but also at a center that is present in the bud side of the nucleus after the initiation of mitosis. This center can be visualized by live microscopy as a punctate body located in close proximity to the nuclear envelope and opposite to the nucleolus. It contains ribosomal DNA (rDNA) and precursors of both 40S and 60S ribosomal subunits. Proteins that actively participate in ribosome synthesis, but not functionally defective variants, accumulate in that site. The formation of this body occurs in the metaphase-to-anaphase transition when discrete regions of rDNA occasionally exit the nucleolus and move into the bud. Collectively, our data unveil the existence of a previously unknown mechanism for preribosome accumulation at the nuclear periphery in budding yeast. We propose that this might be a strategy to expedite the delivery of ribosomes to the growing bud. This work was supported by the Spanish Ministry of Economy and Competitiveness (BFU2014-52729-P to M.D.; SAF2015-64556-R and CB16/12/00351 to X.R.B.), the Junta of Castilla y León (CSI049U16), Worldwide Cancer Research (14-1248 to X.R.B.) and the Ramón Areces Foundation. G.M. and B.N. have been supported by graduate student contracts from the University of Salamanca and Santander Bank. S.G.G. has been supported by a contract on the BFU2014-52729-P grant. Spanish funding to M.D. and X.R.B. is partially contributed by the European Regional Development Fund. |
| Databáze: | OpenAIRE |
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