Histo-Immunological Detection of Collagen Fractions
| Autor: | O. Vilar, J. Barquet, Mercedes A. Paz, O. W. Davidson, R. E. Mancini |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
Dense connective tissue
Globulin Guinea Pigs Fluorescent Antibody Technique Connective tissue Matrix (biology) Antibodies Chemistry Techniques Analytical Poultry General Biochemistry Genetics and Molecular Biology Glycosaminoglycan chemistry.chemical_compound medicine Animals Fluorescein isothiocyanate Chromatography biology Chemistry Immunochemistry Research Cartilage Cell Biology Lagomorpha Molecular biology medicine.anatomical_structure Sephadex biology.protein Collagen Rabbits |
| Zdroj: | Experimental Biology and Medicine. 118:346-350 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-118-29838 |
| Popis: | SummaryThe soluble and insoluble fractions of collagen from chicken leg tendon were used as antigens to induce corresponding antibodies in adult rabbits and guinea pigs. Antibodies were checked immuno-sero-logically and the globulins or its gamma fraction labeled with fluorescein isothiocyanate and purified with Sephadex. Adequately controlled Coons' technique was used in order to detect the presence of the antigen in different types of connective tissue of adult chickens. It was observed: 1) as previously demonstrated, there was cross reaction between the neutral-soluble and the citrate-soluble fractions, whereas a direct reaction occurred with the insoluble collagen fraction, 2) the fluorescent antibodies against these different fractions reacted similarly with the collagen fibers and bundles but not with reticulin and elastic fibers, mucopolysaccharides, basement membranes, nor with matrix of cartilage and bone, 3) intensity of fluorescence was higher with the fibers of dense connective tissue as compa... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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