Comparison of dynamic contrast-enhanced magnetic resonance imaging and contrast-enhanced ultrasound for evaluation of the effects of sorafenib in a rat model of hepatocellular carcinoma
| Autor: | James A. Bankson, Charles V. Kingsley, Jennifer M. Mitchell, Asif Rashid, Rony Avritscher, Houra Taghavi, Adeeb A. Minhaj, Kiersten L. Maldonado, Andrea Cortes, Nina M. Muñoz, Urszula Polak, Joe Ensor |
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| Rok vydání: | 2018 |
| Předmět: |
Sorafenib
Male Carcinoma Hepatocellular Biomedical Engineering Biophysics Contrast Media Vascular permeability Permeability Capillary Permeability Necrosis Cell Line Tumor medicine Biomarkers Tumor Image Processing Computer-Assisted Pimonidazole Animals Radiology Nuclear Medicine and imaging skin and connective tissue diseases Hypoxia medicine.diagnostic_test Neovascularization Pathologic business.industry Ultrasound Liver Neoplasms Magnetic resonance imaging medicine.disease Magnetic Resonance Imaging digestive system diseases Rats Disease Models Animal Hepatocellular carcinoma Nuclear medicine business Perfusion medicine.drug Contrast-enhanced ultrasound |
| Zdroj: | Magnetic resonance imaging. 57 |
| ISSN: | 1873-5894 |
| Popis: | Objectives To compare the accuracy of contrast-enhanced ultrasound (CEUS) and Dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) for the assessment of changes in tissue vascularization as result of sorafenib treatment in a rat model of hepatocellular carcinoma (HCC). Methods Male Buffalo rats with orthotopic liver tumors treated daily with 7.5 mg/kg sorafenib via oral gavage for 2 weeks (n = 9) were subject to DCE-MRI and CEUS 2 weeks after tumor implantation - right before treatment initiation - and also after treatment completion - right before tumor harvest. Untreated animals (n = 10) were used as control. Tumor tissue sections were stained for hematoxylin-eosin, pimonidazole, and CD34 for quantitative assessment of necrosis, hypoxia, and microvessel density (MVD), respectively. Results Of all the DCE-MRI parameters that were evaluated, only volume transfer constant (Ktrans) measurements were significantly lower in sorafenib-treated tumors (0.18 vs 0.33 min−1, p 0.05). Conclusions Sorafenib-induced reduction in vascular permeability in this preclinical model of HCC is detected more accurately through DCE-MRI than CEUS, and DCE-MRI parameters strongly correlate with histopathological changes in tissue vascularization and tissue necrosis. |
| Databáze: | OpenAIRE |
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