| Autor: |
Ho-Kai Huang, Lin, Yi-Hsin, Heng-Ai Chang, Yi-Shyun Lai, Ying-Chi Chen, Soon-Cen Huang, Chou, Cheng-Yang, Wen-Tai Chiu |
| Rok vydání: |
2020 |
| DOI: |
10.6084/m9.figshare.11880444 |
| Popis: |
Additional file 3 : Fig. S3. Effect of SOCE inhibitors on Ca2+ elevation in IGROV1-WT cells. a Pre-incubation of IGROV1-WT cells with 2 μM fura-2/AM and SOCE inhibitors (2 μΜ SKF-96365, 10 μΜ YM-58483, 0.1 μM 2-APB) at 37 °C for 30 min for cytosolic Ca2+ measurement using a single-cell fluorimeter. Depletion of ER lumen-resident Ca2+ was induced by treating cells in Ca2+-free buffer with 2 μM thapsigargin for 10 min. Representative tracings show the subsequent elevation of Ca2+, indicating that SOCE occurred during the exchange of Ca2+-free buffer to 2 mM Ca2+ buffer for 5 min. The data in representative curves for the measurement of SOCE from three independent experiments. b SOCE-mediated total Ca2+ elevation was calculated from area under the curve. a.u., arbitrary unit. Each bar represents mean ± SEM from at least 120 cells. *: significant difference between cells treated with SOCE inhibitors and DMSO control (Ctrl). ***: p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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