Subarachnoid hemorrhage in rats - Visualizing blood distribution in vivo using gadolinium-enhanced magnetic resonance imaging: Technical note
| Autor: | Gregor Kasprian, Hendrik Jan Ankersmit, Elisabeth Simader, Lucian Beer, Thomas H. Helbich, Thomas Raunegger, Thomas Haider, Lubos Budinsky, Philipp Hacker, Romana Höftberger, Camillo Sherif |
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| Přispěvatelé: | Beer, Lucian [0000-0003-4388-7580], Apollo - University of Cambridge Repository |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Subarachnoid hemorrhage Gadolinium Variable time Preclinical model of SAH chemistry.chemical_element Preclinical MRI Subarachnoid haemorrhage (SAH) Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine In vivo Medicine Distribution (pharmacology) Animals cardiovascular diseases medicine.diagnostic_test business.industry General Neuroscience FOS: Clinical medicine Neurosciences Magnetic resonance imaging Technical note Subarachnoid Hemorrhage medicine.disease Magnetic Resonance Imaging nervous system diseases Rats Disease Models Animal 030104 developmental biology chemistry Stereotactic injection Feasibility Studies business Nuclear medicine 030217 neurology & neurosurgery |
| Zdroj: | Journal of neuroscience methods. 325 |
| ISSN: | 1872-678X |
| Popis: | Background The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment. New Method We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up. Results Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images. Comparison with Existing Methods We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model. Conclusion This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models. |
| Databáze: | OpenAIRE |
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