Suppression of Citrus Melanose on the Leaves Treated with Rhizobacterial Strains after Inoculation with Diaporthe citri
Autor: | Yun Jung Ko, So Young Kang, Yong Chull Jeun |
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Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
Burkholderia gladioli
biology Inoculation Pseudomonas Biological pest control Diaporthe citri food and beverages Plant Science biology.organism_classification Biochemistry lcsh:S1-972 Environment-friendly Fungicide Horticulture Internal transcribed spacer Bio-fungicide Biological control lcsh:Agriculture (General) Agronomy and Crop Science Molecular Biology Pathogen Bacteria Biotechnology |
Zdroj: | Research in Plant Disease, Vol 18, Iss 4, Pp 331-337 (2012) |
ISSN: | 2233-9191 1598-2262 |
Popis: | Citrus melanose is one of important disease in citrus cultivation, reducing quality of citrus fruits and resulting in economic loss. Like other diseases in citrus, melanose was mainly protected by chemical fungicide in the field. Recently, alternative method of disease control is highly required due to the side effect of the chemicals. In this study four rhizobacterial strains TRH423-3, MRL408-3, THJ609-3, and TRH415-2 are selected by dual-culture testing its antifungal activity against Diaporthe citri causing citrus melanose. To investigate the protection efficacy of the selected rhizobacterial strains to citrus melanose, the bacteria were pre-treated on citrus leaves following inoculation with melanose pathogen. Pre-treatment with all selected rhizobacterial strains showed disease suppression in which the levels of protection rates were different by the rhizobacterial strains. Additional treatment with the rhizobacterial strains after the pathogen inoculation enhanced protection rates in all cases. The strain MRL408-3 and TRH423-3 were identified as Burkholderia gladioli, TRH415-2 as Pseudomons fluorescens and THJ609-3 as Pseudomonas pudia as a result of analyzing the internal transcript spaces of the rhizobacterial strains rDNA. The selected rhizobacterial strains may be valuable as biological control agents in the environment-friendly citrus farm in which chemical application is limited. |
Databáze: | OpenAIRE |
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