Evaluation of polylactic acid nanoparticles safety using Drosophila model
| Autor: | Bernard Verrier, Sophie Legaz, Christophe Terzian, Claire Lethias, Jean-Yves Exposito, Barbara Viginier |
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| Přispěvatelé: | Infections Virales et Pathologie Comparée - UMR 754 (IVPC), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, European Commission FP7 ADITEC program [HEALTH-F4-2011-280873], FP7 CutHIVac [HEALTH-241904], Fondation Pierre Berge-Yves Saint-Laurent, ANR (grant ANR PECSDDeli and Euronanomed iNanoDCs), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE)-Université Claude Bernard Lyon 1 (UCBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
[SDV]Life Sciences [q-bio] Nanoparticle Biocompatible Materials Toxicology chemistry.chemical_compound Polylactic acid Cytotoxicity Drug Carriers Drug Carriers/chemistry/*toxicity Cell Cycle Nanoparticles/chemistry/*toxicity Flow Cytometry Drosophila melanogaster Larva Drug delivery Drug Drug carrier Cell Survival/drug effects sub-lethal concentration Materials science oral delivery Cell Survival Surface Properties Polyesters Biomedical Engineering Nanotechnology Real-Time Polymerase Chain Reaction Cell Line Dose-Response Relationship 03 medical and health sciences Reactive Oxygen Species/metabolism Toxicity Tests Animals Particle Size Cell Proliferation Drosophila Dose-Response Relationship Drug Cell growth Polyesters/chemistry/*toxicity Cell Cycle/*drug effects Biocompatible Materials/chemistry/*toxicity High-Throughput Screening Assays 030104 developmental biology chemistry Cell culture Cell Proliferation/*drug effects Biophysics Nanoparticles Nanocarriers Reactive Oxygen Species PLA nanoparticle |
| Zdroj: | Nanotoxicology Nanotoxicology, 2016, 10 (8), pp.1136-43. ⟨10.1080/17435390.2016.1181806⟩ |
| Popis: | Cytotoxicity of nanoparticles and their sub-lethal effect on cell behavior and cell fate are a high topic of studies in the nanomaterial field. With an explosion of nanoparticle types (size, shape, polarity, stiffness, composition, etc.), Drosophila has become an attractive animal model for high throughput analysis of these nanocarriers in the drug delivery field with applications in cancer therapy, or simply to generate a fast and complete cytotoxic study of a peculiar nanoparticle. In respect to that, we have conducted an in cellulo study of poly(lactic acid) (PLA) nanoparticle cytotoxicity, and determined that near lethal nanoparticle doses, oxidative stress as well as P53 and ATP pathways may lead to cell cycle arrest at G1, and ultimately to cell death. Neither viability nor the development of Drosophila larvae are affected by the ingestion of PLA nanoparticles at sub-lethal concentrations. Drosophila will be a useful model to study PLA and PLA-modified nanoparticle toxicity, and nanoparticle fate after ingestion. |
| Databáze: | OpenAIRE |
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