Regulation of the expression of epidermal keratinocyte proliferation and differentiation by vitamin A analogs
| Autor: | Cynthia L. Marcelo, K. C. Madison |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Time Factors
medicine.drug_class Cellular differentiation Retinoic acid Tretinoin Dermatology Biology Mice chemistry.chemical_compound Keratin medicine Animals Dimethyl Sulfoxide Retinoid Isotretinoin Vitamin A Cells Cultured Skin chemistry.chemical_classification Dose-Response Relationship Drug Epidermis (botany) Cell growth Retinol Cell Differentiation General Medicine Molecular biology medicine.anatomical_structure Biochemistry chemistry Etretinate Keratinocyte Cell Division |
| Zdroj: | Archives of Dermatological Research. 276:381-389 |
| ISSN: | 1432-069X 0340-3696 |
| DOI: | 10.1007/bf00413359 |
| Popis: | A number of vitamin A analogs (retinoids) were used to manipulate the growth of epidermal keratinocytes in culture. The retinoids used were the TMMP analog of ethyl retinoate (Ro 10-9359), 13-cis retinoic acid, all trans retinoic acid and retinol (trans). These were added to primarily neonatal mouse epidermal keratinocyte cultures that proliferate, stratify, and differentiate over 2-3 weeks. [3H]Tdr labeling technics were used to quantitate proliferation. A histologic stain, and a four buffer protein extraction protocol, used in conjunction with polyacrylamide gel electrophoresis and fluorographic technics, were used to assess the differentiation of the cultures. Our results showed that all of the vitamin A analogs we tested inhibited keratinocyte proliferation. Quantitation of specific differentiation proteins showed that Ro 10-9359 and 13-cis retinoic acid partially inhibited the differentiation of the cultures. The Ro 10-9359 retinoid was unusual in that it increased the synthesis of keratohyalin granule-related proteins. These studies showed that inhibition of basal cell proliferation did not result in the obligatory expression of cell differentiation and that at least one of the events that is a part of epidermal keratinocyte differentiation can be separately controlled. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink