VavCre transgenic mice: a tool for mutagenesis in hematopoietic and endothelial lineages
| Autor: | Georgiades, Pantelis, Ogilvy, S., Duval, H., Licence, D. R., Charnock-Jones, D. S., Smith, S. K., Print, C. G. |
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| Přispěvatelé: | Georgiades, Pantelis [0000-0002-5538-3163] |
| Rok vydání: | 2002 |
| Předmět: |
Somatic cell
genotype polymerase chain reaction beta galactosidase complementary DNA animal cell fluorescence activated cell sorting Mice Endocrinology immunocytochemistry Genes Reporter Leukocytes endothelium cell Transgenes Hematopoietic Regulation of gene expression Oncogene Proteins Recombination Genetic genetic recombination article vav Cre gene expression regulation Flow Cytometry reporter gene medicine.anatomical_structure priority journal histochemistry mutagenesis Genetically modified mouse Cell type Transgene Mutagenesis (molecular biology technique) Cre recombinase Mice Transgenic Biology hematopoietic cell Endothelial Viral Proteins Genetics medicine Animalia Mus musculus Animals Cell Lineage Endothelium cre recombinase Proto-Oncogene Proteins c-vav mouse nonhuman Integrases nucleotide sequence Cell Biology Hematopoietic Stem Cells Molecular biology transgenic mouse Gene Expression Regulation Mutagenesis Bone marrow Murinae |
| Zdroj: | Genesis |
| ISSN: | 1526-954X |
| Popis: | Cre transgenic mice can be used to delete gene sequences flanked by loxP sites in specific somatic tissues. We have generated vavCre transgenic mice, which can be used to inactivate genes specifically in adult hematopoietic and endothelial cells. In these animals, a Cre transgene is expressed under control of murine vav gene regulatory elements. To assess their usefulness, vavCre transgenic mice were bred with R26R mice, which express a lacZ reporter gene only in cells where Cre-mediated recombination has occurred. VavCre/R26R double-heterozygous offspring were analyzed by β-galactosidase histochemistry and flow cytometry. VavCre-mediated recombination occurred in most hematopoietic cells of all hematopoietic organs, including the hematopoietic progenitor-rich bone marrow. Recombination also occurred In most endothelial and germ cells, but only rarely in other cell types. The recombination in both hematopoietic and endothelial lineages may partly reflect their putative shared ontogeny and provides a unique tool for simultaneous pan-hematopoietic and endothelial mutagenesis. © 2002 Wiley-Liss, Inc. 34 251 256 Cited By :74 |
| Databáze: | OpenAIRE |
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