Temporal and spatial expression of CCN genes in zebrafish
| Autor: | Stephanie A. Balow, Cynthia F. Bartels, Michael To, Matthew L. Warman, Patricia A. Conrad, Tomas Marques, Yukio Nakamura, Carol A. Fernando |
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| Rok vydání: | 2010 |
| Předmět: |
Cellular differentiation
Molecular Sequence Data In situ hybridization CTGF connective tissue growth factor CCN Intercellular Signaling Proteins 03 medical and health sciences Peixos zebra -- Genètica Nov nephroblastoma overexpressed Cyr61 cysteine rich protein 61 Animals WISP Wnt-1 inducible signaling pathway protein Zebrafish Gene Cysteine rich protein 61 Adaptor Proteins Signal Transducing 030304 developmental biology 0303 health sciences Base Sequence biology fungi 030302 biochemistry & molecular biology Connective Tissue Growth Factor Cell Differentiation Zebrafish Proteins Patterns & Phenotypes biology.organism_classification Molecular biology Cell biology CTGF Genes CYR61 Cyr61 Proteïnes Function (biology) Developmental Biology |
| Zdroj: | Developmental Dynamics Recercat. Dipósit de la Recerca de Catalunya instname |
| ISSN: | 1058-8388 |
| DOI: | 10.1002/dvdy.22279 |
| Popis: | The six mammalian CCN genes (Cyr61, CTGF, Nov, WISP1, WISP2, WISP3) encode a family of secreted, cysteine-rich, multimodular proteins having roles in cell proliferation, adhesion, migration, and differentiation during embryogenesis, wound healing, and angiogenesis. We used bioinformatics to identify 9 CCN genes in zebrafish (zCCNs), 6 of which have not been previously described. When compared with mammalian CCN family members, 3 were paralogs of Cyr61, 2 of CTGF, 2 of WISP1, 1 of WISP2, and 1 of WISP3. No paralog of Nov was found. In situ hybridization was performed to characterize the sites of expression of the zCCNs during early zebrafish development. zCCNs demonstrated both unique and overlapping patterns of expression, suggesting potential division of labor between orthologous genes and providing an alternate approach to gene function studies that will complement studies in mammalian models. Developmental Dynamics 239:1755–1767, 2010. © 2010 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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