A linear plasmid truncation induces unidirectional flagellar phase change in H:z66 positive Salmonella Typhi
| Autor: | Sally Whitehead, Tim Perkins, Kathryn E. Holt, Ian Goodhead, Jonathan Hardy, Bruce A. D. Stocker, Gordon Dougan, Stephen Baker |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Sequence analysis
Inverted repeat Biology Salmonella typhi Microbiology 03 medical and health sciences Plasmid Bacterial Proteins Molecular Biology Gene Research Articles Sequence Deletion 030304 developmental biology Palindromic sequence Phase variation Genetics Antigens Bacterial 0303 health sciences 030306 microbiology Sequence Analysis DNA Molecular biology Repressor Proteins Flagella biology.protein bacteria Flagellin Plasmids |
| Zdroj: | Molecular Microbiology |
| ISSN: | 0950-382X |
| Popis: | The process by which bacteria regulate flagellar expression is known as phase variation and in Salmonella enterica this process permits the expression of one of two flagellin genes, fliC or fljB, at any one time. Salmonella Typhi (S. Typhi) is normally not capable of phase variation of flagellar antigen expression as isolates only harbour the fliC gene (H:d) and lacks an equivalent fljB locus. However, some S. Typhi isolates, exclusively from Indonesia, harbour an fljB equivalent encoded on linear plasmid, pBSSB1 that drives the expression of a novel flagellin named H:z66. H:z66+S. Typhi isolates were stimulated to change flagellar phase and genetically analysed for the mechanism of variation. The phase change was demonstrated to be unidirectional, reverting to expression from the resident chromosomal fliC gene. DNA sequencing demonstrated that pBSSB1 linear DNA was still detectable but that these derivatives had undergone deletion and were lacking fljA(z66) (encoding a flagellar repressor) and fljB(z66). The deletion end-point was found to involve one of the plasmid termini and a palindromic repeat sequence within fljB(z66), distinct to that found at the terminus of pBSSB1. These data demonstrate that, like some Streptomyces linear elements, at least one of the terminal inverted repeats of pBSSB1 is non-essential, but that a palindromic repeat sequence may be necessary for replication. |
| Databáze: | OpenAIRE |
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