Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis

Autor: Jacob F. Warner, Aldine R. Amiel, Solène Ferreira, Karine Nedoncelle, Hereroa Johnston, Eric Röttinger
Přispěvatelé: Institut de Recherche sur le Cancer et le Vieillissement (IRCAN), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Röttinger, Eric, Université Nice Sophia Antipolis (1965 - 2019) (UNS)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
food.ingredient
Transcription
Genetic
sea anemone
regeneration
wound healing
tissue tracking
marine invertebrate
Nematostella vectensis
Nematostella
Sea anemone
Fluorescence
Article
Catalysis
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
0302 clinical medicine
food
In vivo
Transcription (biology)
[SDV.BDD] Life Sciences [q-bio]/Development Biology
Animals
Physical and Theoretical Chemistry
Molecular Biology
Bilateria
lcsh:QH301-705.5
[SDV.BDD]Life Sciences [q-bio]/Development Biology
Spectroscopy
Cell Proliferation
030304 developmental biology
Regulation of gene expression
0303 health sciences
Microscopy
Confocal
biology
Organic Chemistry
Temperature
General Medicine
Anatomy
Regenerative process
biology.organism_classification
Computer Science Applications
Cell biology
Sea Anemones
Gene Expression Regulation
lcsh:Biology (General)
lcsh:QD1-999
Wound healing
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, MDPI, 2015, 16 (12), pp.28449-28471. ⟨10.3390/ijms161226100⟩
International Journal of Molecular Sciences, Vol 16, Iss 12, Pp 28449-28471 (2015)
International Journal of Molecular Sciences; Volume 16; Issue 12; Pages: 28449-28471
Volume 16
Issue 12
Pages 28449-28471
International Journal of Molecular Sciences, 2015, 16 (12), pp.28449-28471. ⟨10.3390/ijms161226100⟩
ISSN: 1661-6596
1422-0067
Popis: International audience; Cnidarians, the extant sister group to bilateria, are well known for their impressive regenerative capacity. The sea anemone Nematostella vectensis is a well-established system for the study of development and evolution that is receiving increased attention for its regenerative capacity. Nematostella is able to regrow missing body parts within five to six days after its bisection, yet studies describing the morphological, cellular, and molecular events underlying this process are sparse and very heterogeneous in their experimental approaches. In this study, we lay down the basic framework to study oral regeneration in Nematostella vectensis. Using various imaging and staining techniques we characterize in detail the morphological, cellular, and global molecular events that define specific landmarks of this process. Furthermore, we describe in vivo assays to evaluate wound healing success and the initiation of pharynx reformation. Using our described landmarks for regeneration and in vivo assays, we analyze the effects of perturbing either transcription or cellular proliferation on the regenerative process. Interestingly, neither one of these experimental perturbations has major effects on wound closure, although they slightly delay or partially block it. We further show that while the inhibition of transcription blocks regeneration in a very early step, inhibiting cellular proliferation only affects later events such as pharynx reformation and tentacle elongation.
Databáze: OpenAIRE
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