Repressed expression of a gene for a basic helix-loop-helix protein causes a white flower phenotype in carnation
Autor: | Nobuhiro Sasaki, Aiko Watanabe, Emilio A. Cano, Emi Okamoto, Masahiro Nishihara, Yoshihiro Ozeki, Keisuke Tasaki, Taira Miyahara, Takanobu Kouno, Akane Totsuka |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Candidate gene transcriptional regulatory factor Plant Science Carnation 01 natural sciences anthocyanin 03 medical and health sciences Genetics Gene chemistry.chemical_classification biology fungi Structural gene variegation food and beverages Note biology.organism_classification Phenotype White (mutation) 030104 developmental biology Enzyme chemistry basic helix-loop-helix carnation Petal Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Breeding Science |
ISSN: | 1347-3735 1344-7610 |
Popis: | In a previous study, two genes responsible for white flower phenotypes in carnation were identified. These genes encoded enzymes involved in anthocyanin synthesis, namely, flavanone 3-hydroxylase (F3H) and dihydroflavonol 4-reductase (DFR), and showed reduced expression in the white flower phenotypes. Here, we identify another candidate gene for white phenotype in carnation flowers using an RNA-seq analysis followed by RT-PCR. This candidate gene encodes a transcriptional regulatory factor of the basic helix-loop-helix (bHLH) type. In the cultivar examined here, both F3H and DFR genes produced active enzyme proteins; however, expression of DFR and of genes for enzymes involved in the downstream anthocyanin synthetic pathway from DFR was repressed in the absence of bHLH expression. Occasionally, flowers of the white flowered cultivar used here have red speckles and stripes on the white petals. We found that expression of bHLH occurred in these red petal segments and induced expression of DFR and the following downstream enzymes. Our results indicate that a member of the bHLH superfamily is another gene involved in anthocyanin synthesis in addition to structural genes encoding enzymes. |
Databáze: | OpenAIRE |
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