Development of Standardized Fetal Progenitor Cell Therapy for Cartilage Regenerative Medicine: Industrial Transposition and Preliminary Safety in Xenogeneic Transplantation

Autor: Sandra Jaccoud, Nathalie Hirt-Burri, Aurélie Ducrot, Salim E Darwiche, Anthony de Buys Roessingh, Katja Nuss, L.A. Applegate, Brigitte von Rechenberg, Wassim Raffoul, Corinne Scaletta, Dominique P. Pioletti, Philippe Abdel-Sayed, Alexis Laurent
Přispěvatelé: University of Zurich, Darwiche, Salim
Rok vydání: 2021
Předmět:
Cartilage
Articular
1303 Biochemistry
Cell Transplantation
fetal progenitor cells
lcsh:QR1-502
Cell- and Tissue-Based Therapy
large animal model
cartilage cell therapy
Bioinformatics
Regenerative Medicine
Regenerative medicine
Biochemistry
lcsh:Microbiology
Article
Cell therapy
03 medical and health sciences
0302 clinical medicine
Fetus
pilot safety study
Animals
Cartilage
Articular/physiology
Fetus/cytology
Goats/embryology
Heterografts
Humans
Models
Animal
Stem Cells/cytology
MACI
cell banking
chorioallantoic membrane model
preclinical research
medicine
1312 Molecular Biology
Organ donation
Progenitor cell
Autologous chondrocyte implantation
Molecular Biology
030304 developmental biology
0303 health sciences
business.industry
Cartilage
Regeneration (biology)
Goats
Stem Cells
030229 sport sciences
10226 Department of Molecular Mechanisms of Disease
3. Good health
Transplantation
medicine.anatomical_structure
570 Life sciences
biology
business
Zdroj: Biomolecules
Biomolecules, Vol 11, Iss 250, p 250 (2021)
Volume 11
Issue 2
Biomolecules, vol. 11, no. 2, pp. 250
ISSN: 2218-273X
DOI: 10.3390/biom11020250
Popis: Diverse cell therapy approaches constitute prime developmental prospects for managing acute or degenerative cartilaginous tissue affections, synergistically complementing specific surgical solutions. Bone marrow stimulation (i.e., microfracture) remains a standard technique for cartilage repair promotion, despite incurring the adverse generation of fibrocartilagenous scar tissue, while matrix-induced autologous chondrocyte implantation (MACI) and alternative autologous cell-based approaches may partly circumvent this effect. Autologous chondrocytes remain standard cell sources, yet arrays of alternative therapeutic biologicals present great potential for regenerative medicine. Cultured human epiphyseal chondro-progenitors (hECP) were proposed as sustainable, safe, and stable candidates for chaperoning cartilage repair or regeneration. This study describes the development and industrial transposition of hECP multi-tiered cell banking following a single organ donation, as well as preliminary preclinical hECP safety. Optimized cell banking workflows were proposed, potentially generating millions of safe and sustainable therapeutic products. Furthermore, clinical hECP doses were characterized as non-toxic in a standardized chorioallantoic membrane model. Lastly, a MACI-like protocol, including hECPs, was applied in a three-month GLP pilot safety evaluation in a caprine model of full-thickness articular cartilage defect. The safety of hECP transplantation was highlighted in xenogeneic settings, along with confirmed needs for optimal cell delivery vehicles and implantation techniques favoring effective cartilage repair or regeneration.
Databáze: OpenAIRE
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