Development of novel monoclonal antibodies directed against catechins for investigation of antibacterial mechanism of catechins
| Autor: | Xiaoguang Zhang, Ken-ichi Honjoh, Tadahiro Ozawa, Kanami Shimatani, Yasuto Suzuki, Koji Yui, Takahisa Miyamoto, Naofumi Shigemune, Motokazu Nakayama, Kitichalermkiat Apisada, Asako Mitani, Yuuki Ueyama |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Staphylococcus aureus medicine.drug_class medicine.medical_treatment 030106 microbiology Epigallocatechin gallate Monoclonal antibody Microbiology Catechin Cell Line 03 medical and health sciences chemistry.chemical_compound Antibody Specificity Flavan Escherichia coli medicine Animals Gallocatechin gallate Amino Acid Sequence Molecular Biology chemistry.chemical_classification Mice Inbred BALB C Base Sequence Antibodies Monoclonal Polyphenols food and beverages Hemocyanin Anti-Bacterial Agents Amino acid 030104 developmental biology chemistry Biochemistry Polyphenol Hemocyanins Myricetin |
| Zdroj: | Journal of Microbiological Methods. 137:6-13 |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/j.mimet.2017.03.014 |
| Popis: | Catechins are major polyphenolic compounds of green tea. To investigate mechanism for antibacterial action of catechins, 11 monoclonal antibodies (MAbs) were raised against a 3-succinyl-epicatechin (EC)-keyhole limpet hemocyanin (KLH) conjugate. Amino acid sequences of variable regions determined for MAbs b-1058, b-1565, and b-2106 confirmed their innovative character. MAb b-1058 strongly interacted with its target substances in the following order of magnitude: theaflavin-3,3'-di-O-gallate (TFDG)>theaflavin-3-O-gallate (TF3G)≥theaflavin-3'-O-gallate (TF3'G)>gallocatechin gallate (GCg)>penta-O-galloyl-β-d-glucose (PGG)>epigallocatechin gallate (EGCg), as determined using surface plasmon resonance (SPR) on MAb-immobilized sensor chips. The affinity profiles of MAbs b-1058 and b-2106 to the various polyphenols tested suggested that flavan skeletons with both carbonyl oxygen and hydroxyl groups are important for this interaction to take place. S. aureus cells treated with EGCg showed green fluorescence around the cells after incubation with FITC-labeled MAb b-1058. The fluorescence intensity increased with increasing concentrations of EGCg. These MAbs are effective to investigate antibacterial mechanism of catechins and theaflavins. |
| Databáze: | OpenAIRE |
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