In vitro biocompatibility of an ultrafine grained zirconium
| Autor: | L. Munuera, José Luis González-Carrasco, Nuria Vilaboa, María L González-Martín, María Teresa Pérez-Prado, Marta Multigner, Ling Jiang, Laura Saldaña, A. Méndez-Vilas |
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| Rok vydání: | 2007 |
| Předmět: |
Materials science
Biocompatibility Cell Survival Surface Properties Biophysics chemistry.chemical_element Biocompatible Materials Bioengineering In Vitro Techniques Biomaterials Calcification Physiologic Osteogenesis Tubulin Cell Line Tumor Materials Testing Alloys Cell Adhesion medicine Humans Nanotopography Particle Size Cells Cultured Cytoskeleton Strengthening mechanisms of materials Titanium Zirconium Osteoblasts Metallurgy Biomaterial Mesenchymal Stem Cells Osteoblast Alkaline Phosphatase Actins Grain size Fibronectins medicine.anatomical_structure chemistry Chemical engineering Mechanics of Materials Microscopy Electron Scanning Ceramics and Composites Severe plastic deformation Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2007.06.015 |
| Popis: | We have investigated a novel ultrafine grained (UFG) Zr obtained by severe plastic deformation (SPD) which resulted in a refinement of the grain size by several orders of magnitude. Compared to conventional Zr, higher hardness values were measured on UFG Zr. Polished surfaces having similar topographical features from both materials were prepared, as assessed by atomic force microscopy (AFM). Surface hydrophobicity of Zr, evaluated by measuring water contact angles, was unaffected by grain size reduction. In vitro biocompatibility was addressed on conventional and UFG Zr surfaces and, for comparative purposes, a polished Ti6Al4 V alloy was also investigated. Cell attachment and spreading, actin and β-tubulin cytoskeleton reorganisation, fibronectin secretion and cellular distribution as well as cell viability were evaluated by culturing human osteoblastic Saos-2 cells on the surfaces. The osteoblastic response to conventional Zr was found to be essentially identical to Ti6Al4V and was not affected by grain size reduction. In order to evaluate the ability of the surfaces to promote osteogenic maturation and bone matrix mineralisation, human mesenchymal cells from bone marrow were switched to the osteoblastic phenotype by incubation in osteogenic induction media. Compared to undifferentiated mesenchymal cells, alkaline phosphatase activity and formation of mineralisation nodules were enhanced to the same extent on both Zr surfaces and Ti6Al4V alloy after induction of osteoblastic differentiation. In summary, improved mechanical properties together with excellent in vitro biocompatibility make UFG Zr a promising biomaterial for surgical implants. © 2007 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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