Evaluation of high-performance liquid chromatography laser-induced fluorescence for serum protein profiling for early diagnosis of oral cancer
| Autor: | Sajan D. George, Ajeetkumar Patil, Vijendra Prabhu, Sujatha Bhat, V. B. Kartha, Keerthilatha M. Pai, Ravikiran Ongole, Santhosh Chidangil, Jayarama K. Shetty, K.S. Choudhari, V. K. Unnikrishnan |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
medicine.medical_specialty Pathology Biomedical Engineering Serum protein Early detection Protein profile Sensitivity and Specificity High-performance liquid chromatography Biomaterials Biomarkers Tumor medicine Humans Laser-induced fluorescence Chromatography High Pressure Liquid Receiver operating characteristic business.industry Lasers Reproducibility of Results Middle Aged Serum samples Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Spectrometry Fluorescence Female Mouth Neoplasms Histopathology business Blood Chemical Analysis |
| Zdroj: | Journal of Biomedical Optics. 15:067007 |
| ISSN: | 1083-3668 |
| DOI: | 10.1117/1.3523372 |
| Popis: | The present work deals with the evaluation of a high-performance liquid chromatography laser-induced fluorescence (HPLC-LIF) technique developed in our laboratory for early detection of oral cancer from protein profiles of body fluids. The results show that protein profiles of serum samples from a given class of samples, say, normal, premalignant, or malignant, are statistically very close to each other, while profiles of members of any class are significantly different from other classes. The performance of the technique is evaluated by the use of sensitivity and specificity pairs, receiver operating characteristic (ROC) analysis, and Youden's Index. The technique uses protein profile differences in serum samples, registered by the HPLC-LIF technique. The study is carried out using serum samples from volunteers diagnosed as normal or premalignant clinically, and as malignant by histopathology. The specificities and sensitivities of the HPLC-LIF method at an ideal threshold (M-distance = 2) for normal, malignant, and premalignant classes are 100, 69.5, and 61.5%, and 86.5, 87.5, and 87.5% respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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