Pharmacokinetics of forsythoside after intravenous administration in beagle dogs
| Autor: | Zhenyu Xuan, Hongguo Lu, Yingying Liu, Rong Shi, Yueming Ma, Tianjiang Sun |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Pharmacology
Male Validation study Chromatography Dose-Response Relationship Drug Chemistry Half-life Reproducibility of Results High-performance liquid chromatography Beagle Dose–response relationship Dogs Pharmacokinetics Area Under Curve Injections Intravenous Protein precipitation Animals Pharmacology (medical) Female Glycosides Quantitative analysis (chemistry) Forsythia Chromatography High Pressure Liquid Drugs Chinese Herbal Half-Life |
| Zdroj: | European journal of drug metabolism and pharmacokinetics. 34(2) |
| ISSN: | 0378-7966 |
| Popis: | A high-performance liquid chromatography with ultraviolet detection method was established and validated for quantification of forsythoside concentrations in dog plasma. Following a single-step protein precipitation with perchloric acid, the forsythoside and internal standard were separated on a reversed-phase C18 column with water-glacial acetic acid-methanol as mobile phase at a flow rate of 1 mL/min with ultraviolet detection at 326 and 278 nm for forsythoside and IS, respectively. The calibration curve for forsythoside was linear over a range of 0.052–13.33 μg/mL with correlation coefficient of 0.999. The within- and between-batch precisions of analysis were |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink