Embryonic Rather than Extraembryonic Tissues Have More Impact on the Development of Placental Hyperplasia in Cloned Mice
Autor: | Noriko Wakisaka, Narumi Ogonuki, Akihiko Ohta, Hiromi Miki, Atsuo Ogura, Kimiko Inoue, J.-M. Kim, M. Mori |
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Rok vydání: | 2009 |
Předmět: |
Male
medicine.medical_specialty Placenta Diseases Somatic cell Cloning Organism Placenta Population Extraembryonic Membranes Mice Transgenic Biology Models Biological Mice Chimera (genetics) Fetus Pregnancy Internal medicine medicine Animals education Cloning education.field_of_study Hyperplasia Chimera Obstetrics and Gynecology Placentation Embryo Embryo Mammalian Embryonic stem cell Cell biology Mice Inbred C57BL Endocrinology medicine.anatomical_structure Reproductive Medicine embryonic structures Female Developmental Biology |
Zdroj: | Placenta. 30:543-546 |
ISSN: | 0143-4004 |
DOI: | 10.1016/j.placenta.2009.03.006 |
Popis: | Somatic cell cloning by nuclear transfer (NT) in mice is associated with hyperplastic placentas at term. To dissect the effects of embryonic and extraembryonic tissues on this clone-associated phenotype, we constructed diploid (2 n ) fused with (⇔) tetraploid (4 n ) chimeras from NT- and fertilization-derived (FD) embryos. Generally, the 4 n cells contributed efficiently to all the extraembryonic tissues but not to the embryo itself. Embryos constructed by 2 n NT ⇔ 4 n FD aggregation developed hyperplastic placentas (0.33 ± 0.22 g) with a predominant contribution by NT-derived cells. Even when the population of FD-derived cells in placentas was increased using multiple FD embryos (up to four) for aggregation, most placentas remained hyperplastic (0.36 ± 0.13 g). By contrast, placentas of the reciprocal combination, 2 n FD ⇔ 4 n NT, were less hyperplastic (0.15 ± 0.02 g). These nearly normal-looking placentas had a large proportion of NT-derived cells. Thus, embryonic rather than extraembryonic tissues had more impact on the onset of placental hyperplasia, and that the abnormal placentation in clones occurs in a noncell-autonomous manner. These findings suggest that for improvement of cloning efficiency we should understand the mechanisms regulating placentation, especially those of embryonic origin that might control the proliferation of trophoblastic lineage cells. |
Databáze: | OpenAIRE |
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