Research article expression of surfactant protein-A and D, and CD9 in lungs of 1 and 30 day old foals
| Autor: | Tara Bocking, Amitoj Singh, Laura E. Johnson, Gurpreet Kaur Aulakh, Baljit Singh, Atul R. Desai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty 040301 veterinary sciences animal diseases Veterinary medicine Horse digestive system Tetraspanin 29 0403 veterinary science Surface-Active Agents 03 medical and health sciences Immune system Western blot biology.animal Macrophages Alveolar parasitic diseases SF600-1100 Animals Medicine Research article Horses Respiratory system Lung 030304 developmental biology 0303 health sciences SP-A Pulmonary Surfactant-Associated Protein A General Veterinary biology medicine.diagnostic_test business.industry SP-D 04 agricultural and veterinary sciences General Medicine respiratory system CD9 Pulmonary Surfactant-Associated Protein D Tetraspanin 3. Good health Surfactant protein A Blot Animals Newborn Foal embryonic structures business Research Article |
| Zdroj: | BMC Veterinary Research, Vol 17, Iss 1, Pp 1-9 (2021) BMC Veterinary Research |
| ISSN: | 1746-6148 |
| Popis: | Background Respiratory diseases are a major cause of morbidity and mortality in the horses of all ages including foals. There is limited understanding of the expression of immune molecules such as tetraspanins and surfactant proteins (SP) and the regulation of the immune responses in the lungs of the foals. Therefore, the expression of CD9, SP-A and SP-D in foal lungs was examined. Results Lungs from one day old (n = 6) and 30 days old (n = 5) foals were examined for the expression of CD9, SP-A, and SP-D with immunohistology and Western blots. Western blot data showed significant increase in the amount of CD9 protein (p = 0.0397) but not of SP-A and SP-D at 30 days of age compared to one day. Immunohistology detected CD9 in the alveolar septa and vascular endothelium but not the bronchiolar epithelium in the lungs of the foals in both age groups. SP-A and SP-D expression was localized throughout the alveolar septa including type II alveolar epithelial cells and the vascular endothelium of the lungs in all the foals. Compared to one day old foals, the expression of SP-A and SP-D appeared to be increased in the bronchiolar epithelium of 30 day old foals. Pulmonary intravascular macrophages were also positive for SP-A and SP-D in 30 days old foals and these cells are not developed in the day old foals. Conclusions This is the first data on the expression of CD9, SP-A and SP-D in the lungs of foals. |
| Databáze: | OpenAIRE |
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