Effect of the combination of several irrigants on dentine surface properties, adsorption of chlorhexidine and adhesion of microorganisms to dentine
| Autor: | Marco Antonio Hungaro Duarte, R. van der Hoeven, M. Jafelicci Jr., Luciano Bachmann, Clóvis Monteiro Bramante, Renato Menezes Silva, Caroline Wichnieski, Ariadne Letra, Talita Tartari |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
CLOREXIDINA Sodium Hypochlorite Surface Properties medicine.medical_treatment Bacterial Adhesion Enterococcus faecalis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Adsorption Candida albicans Materials Testing medicine Animals Peracetic Acid General Dentistry Saline Edetic Acid Chelating Agents Root Canal Irrigants biology Chemistry Chlorhexidine Etidronic Acid 030206 dentistry Adhesion biology.organism_classification 030104 developmental biology Attenuated total reflection Sodium hypochlorite Dentin Cattle Wetting medicine.drug Nuclear chemistry |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0143-2885 |
| DOI: | 10.1111/iej.12960 |
| Popis: | AIM To investigate the effects of combinations of several irrigants on the roughness and wettability of dentine, adhesion of Enterococcus faecalis and Candida albicans and adsorption of chlorhexidine (CHX) to the dentine. METHODOLOGY Bovine dentine samples were prepared and their surface roughness standardized. The samples were distributed in groups (n = 10) and subjected to one of the following irrigation protocols: G1 - saline solution; G2 - sodium hypochlorite (NaOCl); G3 - NaOCl + ethylenediaminetetraacetic acid (EDTA); G4 - NaOCl + peracetic acid (PAA); G5 - NaOCl + 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP); G6 - NaOCl + EDTA + CHX; G7 - NaOCl + PAA + CHX; G8 - NaOCl + HEDP + CHX; and G9 - mixture of NaOCl + HEDP. After treatments, roughness and wettability were measured. In order to evaluate the adhesion of microorganisms to dentine, new dentine samples were prepared and after 2 h of contact with the microorganisms, were analysed using a confocal laser scanning microscope and the number of microorganisms adhering to the surfaces were determined. Absorption spectra were collected by attenuated total reflectance of Fourier-transform infrared spectroscopy before and after immersion of other dentine samples in each solution of G6, G7 and G8 and in a solution of 2% CHX at various time intervals. The areas of the band associated with CHX with the peak at 1492 cm-1 were calculated between 1479 and 1500 cm-1 of the spectral range. The data obtained in all experiments were subjected to one-way ANOVA (α 0.05), whilst EDTA and PAA did (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text