Renal toxicogenomic response to chronic uranyl nitrate insult in mice
| Autor: | Jacques Demaille, Marie-Catherine Romey, Magali Taulan, Olivia Delissen, François Paquet, Christophe Maubert |
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| Přispěvatelé: | Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de radiotoxicologie et radiobiologie expérimentale (LRTOX), Institut de Radioprotection et de Sûreté Nucléaire (IRSN) |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
long-term exposure
creatinine blood level Health Toxicology and Mutagenesis oral drug administration Administration Oral 010501 environmental sciences Kidney Inbred C57BL 01 natural sciences SAGE chemistry.chemical_compound Mice Random Allocation cell metabolism Gene expression oxidative stress animal Serial analysis of gene expression primer DNA Oligonucleotide Array Sequence Analysis Sequence Tagged Sites 0303 health sciences C57BL mouse Reverse Transcriptase Polymerase Chain Reaction drug effect creatinine article Articles gene expression regulation toxicogenetics Toxicogenomics 3. Good health Real-time polymerase chain reaction medicine.anatomical_structure priority journal real time polymerase chain reaction Administration Uranyl Nitrate signal transduction Oral medicine.medical_specialty animal experiment hydrogen peroxide Biology randomization Nephrotoxicity long term exposure reverse transcription polymerase chain reaction 03 medical and health sciences ribosome protein Internal medicine medicine gene expression profiling Animalia Animals controlled study mouse 030304 developmental biology 0105 earth and related environmental sciences DNA Primers [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health nonhuman gene expression profiles drinking water Public Health Environmental and Occupational Health DNA microarray Molecular biology Mice Inbred C57BL Endocrinology sequence tagged site Uranyl nitrate chemistry gene expression RNA [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie serial analysis of gene expression metabolism [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Toxicant |
| Zdroj: | Environmental Health Perspectives Environmental Health Perspectives, National Institute of Environmental Health Sciences, 2004, 112 (16), pp.1628-1635. ⟨10.1289/txg.7296⟩ |
| ISSN: | 0091-6765 1552-9924 |
| DOI: | 10.1289/txg.7296⟩ |
| Popis: | International audience; Although the nephrotoxkity of uranium has been established through numerous animal studies, relatively little is known about the effects of long-term environmental uranium exposure. Using a combination of conventional biochemical studies and serial analysis of gene expression (SAGE), we examined the renal responses to uranyl nitrate (UN) chronic exposure. Renal uranium levels were significantly increased 4 months after ingestion of uranium in drinking water. Creatinine levels in serum were slightly but significantly increased compared with those in controls. Although no further significant differences in other parameters were noted, substantial molecular changes were observed in toxicogenomic profiles. UN induced dramatic alterations in expression levels of more than 200 genes, mainly up-regulated, including oxidative-response-related genes, genes encoding for cellular metabolism, ribosomal proteins, signal transduction, and solute transporters. Seven differentially expressed transcripts were confirmed by real-time quantitative polymerase chain reaction. In addition, significantly increased peroxide levels support the implication of oxidative stress in UN toxicant response. This report highlights the potential of SAGE for the discovery of novel toxicant-induced gene expression alterations. Here, we present, for the first time, a comprehensive view of renal molecular events after uranium long-term exposure. |
| Databáze: | OpenAIRE |
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