AN EFFECTIVE METHOD OF RNA ISOLATION FROMFallopia multifloraTUBEROUS ROOTS
| Autor: | Xue-mei Tan, Lei Chen, Shu-Jing Sheng, Hong-qing Li, Shu-jin Zhao, Yan-Jing Shen |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Electrophoresis
Agar Gel biology Plant Extracts cDNA library RNA CDNA Library Construction General Medicine Blotting Northern biology.organism_classification Plant Roots Polygonaceae Biochemistry law.invention Real-time polymerase chain reaction RNA Plant Fallopia multiflora law Botany Northern blot RNA extraction Polymerase chain reaction Gene Library Biotechnology |
| Zdroj: | Preparative Biochemistry and Biotechnology. 42:87-96 |
| ISSN: | 1532-2297 1082-6068 |
| DOI: | 10.1080/10826068.2011.566297 |
| Popis: | To isolate high-quality total RNA from Fallopia multiflora tuberous roots is difficult because of the presence of high levels of carbohydrates, phenolics, and other secondary metabolites. Since several procedures specialized for RNA isolation from polysaccharides and phenols rich tissues have resulted in poor yields, in this study, we developed a modified protocol that was derived from the traditional CTAB method. The protocol was able to produce high-quality and intact RNA from the tuberous roots of F. multiflora. The yield of total RNA was more than 0.15 mg/g fresh weight, with an A260/A280 ratio of 1.9-2.0. The obtained RNA was of sufficient quality and suitable for downstream application such as reverse-transcription polymerase chain reaction (RT-PCR), Northern hybridization, and cDNA library construction. The protocol may also have wider applicability for total RNA isolation from other plant species with tuberous roots. |
| Databáze: | OpenAIRE |
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