Knock-down of heat-shock protein 90 and isocitrate lyase gene expression reduced root-knot nematode reproduction
| Autor: | Maria Fatima Grossi-de-Sa, Isabela Tristan Lourenço-Tessutti, Diogo Martins-de-Sa, Maria Cristina Mattar da Silva, Janice de Almeida-Engler, Rodrigo da Rocha Fragoso, Antônio Américo Barbosa Viana, R. C. Togawa, José Dijair Antonino de Souza Júnior, Regina M. D. G. Carneiro, João Batista |
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| Přispěvatelé: | Universidade de Brasília (UnB), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Universidade Federal de Minas Gerais, Universidade Católica de Brasília (UCB), Brazilian Agricultural Research Corporation (Embrapa), National Council for Science and Technology (CNPq), Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES), CAPES/COFECUB |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Nicotiana tabacum
[SDV]Life Sciences [q-bio] Soybean cyst-nematode Gene Expression Plant Science Genetically modified crops Plant Roots RNA interference Tobacco Botany Meloidogyne incognita Animals Root-knot nematode Caenorhabditis-elegans Tylenchoidea Gene Heat-Shock Proteins Plant Diseases RNA Double-Stranded 2. Zero hunger Genetics biology Reproduction fungi food and beverages Helminth Proteins Isocitrate lyase Plants Genetically Modified biology.organism_classification Isocitrate Lyase RNA silencing Mc16d10l confers resistance [SDE]Environmental Sciences Female RNA Interference Agronomy and Crop Science Plant-parasitic nematode |
| Zdroj: | Phytopathology Phytopathology, American Phytopathological Society, 2015, 105 (5), pp.628-637. ⟨10.1094/PHYTO-09-14-0237-R⟩ |
| ISSN: | 0031-949X |
| DOI: | 10.1094/PHYTO-09-14-0237-R⟩ |
| Popis: | International audience; Crop losses caused by nematode infections are estimated to be valued at USD 157 billion per year. Meloidogyne incognita, a root-knot nematode (RKN), is considered to be one of the most important plant pathogens due to its worldwide distribution and the austere damage it can cause to a large variety of agronomically important crops. RNA interference (RNAi), a gene silencing process, has proven to be a valuable biotechnology alternative method for RKN control. In this study, the RNAi approach was applied, using fragments of M. incognita genes that encode for two essential molecules, heat-shock protein 90 (HSP90) and isocitrate lyase (ICL). Plant-mediated RNAi of these genes led to a significant level of resistance against M. incognita in the transgenic Nicotiana tabacum plants. Bioassays of plants expressing HSP90 dsRNA demonstrated a delay in gall formation and up to 46% reduction in eggs compared with wild-type plants. A reduction in the level of HSP90 transcripts was observed in recovered eggs from plants expressing dsRNA, indicating that gene silencing persisted and was passed along to first progeny. The ICL knock-down had no clear effect on gall formation but resulted in up to 77% reduction in egg oviposition compared with wild-type plants. Our data suggest that both genes may be involved in RKN development and reproduction. Thus, in this paper, we describe essential candidate genes that could be applied to generate genetically modified crops, using the RNAi strategy to control RKN parasitism. |
| Databáze: | OpenAIRE |
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