TCR alpha/beta and TCR gamma/delta CD4-/CD8- HLA-DR alloreactive CTL clones do not use Fas/Fas ligand pathway to lyse their specific target cells
| Autor: | Caroline Flament, Catherine Gaudin, C Asselin-Paturel, Salem Chouaib, Fathia Mami-Chouaib |
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| Rok vydání: | 1996 |
| Předmět: |
Cytotoxicity
Immunologic CD8 Antigens Receptors Antigen T-Cell alpha-beta Immunology chemical and pharmacologic phenomena Immunofluorescence Ligands Fas ligand Cell Line MHC class I medicine Immunology and Allergy Humans fas Receptor Cytotoxicity biology medicine.diagnostic_test Receptors Antigen T-Cell gamma-delta General Medicine Fas receptor Molecular biology Clone Cells Cytolysis Perforin CD4 Antigens biology.protein CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Human immunology. 51(1) |
| ISSN: | 0198-8859 |
| Popis: | The expression of Fas Ligand (FasL) on human TCRalpha/beta and TCRgamma/delta CD4 − /CD8 − MHC class II-alloreactive clones and Fas/FasL-mediated cytotoxicity were investigated. These clones mediated a HLA-DR2-restricted cytotoxicity toward E418 B cell line (Fas + ). Northern blot analysis demonstrated that all the clones expressed Fast mRNA upon stimulation with E418 specific target. FasL surface expression was detected by immunofluorescence analysis using Fas-Fc soluble protein as well as anti-FasL polyclonal antibodies. Cytotoxicity experiments performed in the presence of anti-Fas, anti-FasL and Fas-Fc molecule indicated that these reagents were unable to inhibit T cell clone mediated lysis toward E418. In addition, when emetine, known to inhibit the induction of Fas-mediated killing, was added during the cytolysis effector phase, no inhibition was observed. These data strongly suggest that Fas/FasL pathway is not involved in this particular T-cell clone-mediated lysis. This cytotoxicity is extracellular Ca 2+ -dependent and is abolished in the presence of EGTA suggesting that it is mainly perforin/granzymebased. |
| Databáze: | OpenAIRE |
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