Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication
Autor: | Tanyaradzwa P. Ndongwe, Vincent Yapo, Maritza Puray-Chavez, Mark Hannink, Mahmoud H. Farghali, Thomas G. Laughlin, Philip R. Tedbury, Dandan Liu, Mary C. Casey, Andrew D. Huber, Stefan G. Sarafianos |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Gene Expression Regulation
Viral lcsh:QR1-502 Biology Virus Replication medicine.disease_cause Article Virus lcsh:Microbiology Cell Line Small hairpin RNA Mice Viral Proteins 03 medical and health sciences Virology Gene expression medicine Animals Humans CRISPR Cells Cultured 030304 developmental biology Hepatitis B virus 0303 health sciences 030306 microbiology RNA virus diseases Hepatitis B RNA Helicase A host factors digestive system diseases 3. Good health Infectious Diseases Viral replication Host-Pathogen Interactions gene expression Microbial Interactions RNA Viral Moloney murine leukemia virus moloney leukemia virus 10 hepatitis B virus RNA Helicases Protein Binding |
Zdroj: | Viruses Volume 11 Issue 7 Viruses, Vol 11, Iss 7, p 651 (2019) |
ISSN: | 1999-4915 |
DOI: | 10.3390/v11070651 |
Popis: | Moloney leukemia virus 10 (MOV10) is an RNA helicase that has been shown to affect the replication of several viruses. The effect of MOV10 on Hepatitis B virus (HBV) infection is not known and its role on the replication of this virus is poorly understood. We investigated the effect of MOV10 down-regulation and MOV10 over-expression on HBV in a variety of cell lines, as well as in an infection system using a replication competent virus. We report that MOV10 down-regulation, using siRNA, shRNA, and CRISPR/Cas9 gene editing technology, resulted in increased levels of HBV DNA, HBV pre-genomic RNA, and HBV core protein. In contrast, MOV10 over-expression reduced HBV DNA, HBV pre-genomic RNA, and HBV core protein. These effects were consistent in all tested cell lines, providing strong evidence for the involvement of MOV10 in the HBV life cycle. We demonstrated that MOV10 does not interact with HBV-core. However, MOV10 binds HBV pgRNA and this interaction does not affect HBV pgRNA decay rate. We conclude that the restriction of HBV by MOV10 is mediated through effects at the level of viral RNA. |
Databáze: | OpenAIRE |
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