Gadolinium Uncouples Mechanical Detection and Osmoreceptor Potential in Supraoptic Neurons
| Autor: | Charles W. Bourque, Stéphane H. R. Oliet |
|---|---|
| Přispěvatelé: | Oliet, Stéphane, Centre for Reserach in Neuroscience, McGill University = Université McGill [Montréal, Canada]-Montreal General Hospital, McGill University Health Center [Montreal] (MUHC)-McGill University Health Center [Montreal] (MUHC) |
| Rok vydání: | 1996 |
| Předmět: |
Male
Vasopressin MESH: Rats Vasopressins Neuroscience(all) MESH: Neurons Gadolinium Gating MESH: Research Support Non-U.S. Gov't Ion Channels Supraoptic nucleus MESH: Osmolar Concentration Cations Animals MESH: Animals [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Mechanotransduction MESH: Cations MESH: Cell Size Ion channel Cell Size Neurons Osmoreceptor Chemistry General Neuroscience Osmolar Concentration Pipette MESH: Mechanoreceptors Anatomy MESH: Ion Channel Gating Water-Electrolyte Balance MESH: Male Rats MESH: Ion Channels MESH: Supraoptic Nucleus MESH: Water-Electrolyte Balance Biophysics [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] MESH: Gadolinium MESH: Vasopressins Ion Channel Gating Mechanoreceptors Supraoptic Nucleus Flux (metabolism) |
| Zdroj: | HAL Neuron Neuron, Elsevier, 1996, 16, pp.175-81 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/s0896-6273(00)80034-3 |
| Popis: | Stretch-sensitive ion channels are ubiquitous, yet evidence of their role in mechanotransduction remains scarce. The presence of stretch-inactivated cation channels in supraoptic neurons is consistent with the osmoreceptor potentials regulating vasopressin release. However, whether osmosensitivity depends on mechanical gating and ion flux through stretch-inactivated channels is unknown. Here we report that changes in channel open probability associated either with modification of pipette pressure or with external osmolality selectively result from variations in closed time. While channel mechanosensitivity and osmotically evoked changes in cell volume are not affected by gadolinium, similar concentrations of the lanthanide inhibit cation permeation through the single channels and macroscopic osmoreceptor potentials. Mechanotransduction through stretch-inactivated channels is therefore necessary for osmoreception in supraoptic neurons. |
| Databáze: | OpenAIRE |
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