Immune Response to Vaccinia Virus Recombinants Expressing Glycoproteins gE, gB, gH, and gL of Varicella-Zoster Virus
| Autor: | Viera Ludvíková, Petr Hainz, Lucie Marešová, L. Kutinová, Šárka Němečková |
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| Rok vydání: | 2001 |
| Předmět: |
herpesviruses
Herpesvirus 3 Human viruses Genetic Vectors Guinea Pigs Gene Expression Vaccinia virus Viral Plaque Assay Biology immunization Antibodies Viral medicine.disease_cause Virus Cell Line Mice Viral Proteins chemistry.chemical_compound Viral Envelope Proteins Antigen vaccine Virology Chlorocebus aethiops medicine antibodies Animals Humans Antigens Viral Glycoproteins Skin Skin Tests Recombination Genetic varicella-zoster virus Membrane Glycoproteins Immunogenicity Varicella zoster virus Complement System Proteins Molecular biology chemistry Immunization Delayed hypersensitivity virus neutralization biology.protein Female Vaccinia Antibody cellular imunity |
| Zdroj: | Virology. 280:211-220 |
| ISSN: | 0042-6822 |
| Popis: | Immunogenicity of Varicella-zoster virus glycoproteins gE, gB, gH, and gL expressed by recombinant vaccinia viruses (VV) separately or simultaneously was determined in mice and guinea pigs by ELISA, Western blotting, radioimmunoprecipitation, plaque reduction assay, and skin test. Single VV-gE and VV-gB recombinants and double VV-gH/gL recombinant elicited specific antibodies with VZV neutralizing activity in mice. Co-expression of gE and gB by one recombinant VV resulted in an increased antibody response in comparison with immunization with single recombinants or their mixtures. Unlike anti-gB and anti-gH/gL antibodies, the gE-specific antibodies had no virus neutralizing activity in absence of complement, and when used alone, they even caused considerable increase of VZV infectious units. Moreover, immune sera containing anti-gE antibodies antagonized complement independent virus-neutralizing activity of anti-gB- and anti-gH/gL-positive sera. The ability to induce delayed hypersensitivity reaction to VZV antigens was observed after immunization of guinea pigs with gE- and/or gB-expressing VVs. |
| Databáze: | OpenAIRE |
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