Novel role for galectin-1 in T-cells under physiological and pathological conditions
| Autor: | Magdolna Deák, Dmytro Demydenko, Enikő Szabó, Julianna Novák, László Kovács, Ágnes Czibula, Éva Monostori, Éva Kriston-Pál, Roberta Fajka-Boja, Ákos Hornung |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Galectin 1 T-Lymphocytes Immunology Intracellular Space Gene Expression Apoptosis Biology Lymphocyte Activation Jurkat cells Cell Line Jurkat Cells Mice Internal medicine medicine Animals Humans Lupus Erythematosus Systemic Immunology and Allergy Autocrine signalling Mice Knockout Systemic lupus erythematosus Wild type Hematology medicine.disease Cell biology Endocrinology Case-Control Studies Galectin-1 Knockout mouse Intracellular |
| Zdroj: | Immunobiology. 220:483-489 |
| ISSN: | 0171-2985 |
| DOI: | 10.1016/j.imbio.2014.10.023 |
| Popis: | Secreted, extracellular galectin-1 (exGal-1) but not intracellular Gal-1 (inGal-1) has been described as a strong immunosuppressive protein due to its major activity of inducing apoptosis of activated T-cells. It has previously been reported that T-cells express Gal-1 upon activation, however its participation in T-cell functions has remained largely elusive. To determine function of Gal-1 expressed by activated T-cells we have carried out a series of experiments. We have shown that Gal-1, expressed in Gal-1-transgenic Jurkat cells or in activated T-cells, remained intracellularly indicating that Gal-1-induced T-cell death was not a result of an autocrine effect of the de novo expressed Gal-1. Rather, a particular consequence of the inGal-1 expression was that T-cells became more sensitive to exGal-1 added either as a soluble protein or bound to the surface of a Gal-1-secreting effector cell. This was also verified when the susceptibility of activated T-cells from wild type or Gal-1 knockout mice to Gal-1-induced apoptosis were compared. Murine T-cells expressing Gal-1 were more sensitive to the cytotoxicity of the exGal-1 than their Gal-1 knockout counterparts. We also conducted a study with activated T-cells from patients with systemic lupus erythematosus (SLE), a disease in which dysregulated T-cell apoptosis has been well described. SLE T-cells expressed lower amounts of Gal-1 than healthy T-cells and were less sensitive to exGal-1. These results suggested a novel role of inGal-1 in T-cells as a regulator of T-cell response to exGal-1, and its likely contribution to the mechanism in T-cell apoptosis deficiency in lupus. |
| Databáze: | OpenAIRE |
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