Microarray comparative genomic hybridization reveals genome-wide patterns of DNA gains and losses in post-Chernobyl thyroid cancer
| Autor: | Somnit Lee, Kenneth J. Kopecky, R. Michael Tuttle, Anatoli F. Tsyb, Mark Aronszajn, Lue Ping Zhao, Doan Nguyen, Chun Cheng, Vladislav Troshin, Paul E. Neiman, Jeffrey J. Delrow, Alexander Abrosimov, Scott Davis, Robert Kimmel |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Neoplasms Radiation-Induced Microarray Adolescent DNA Mutational Analysis Biophysics Gene Dosage Biology Genome chemistry.chemical_compound Complementary DNA medicine Prevalence Humans Radiology Nuclear Medicine and imaging Thyroid Neoplasms Child Thyroid cancer In Situ Hybridization Fluorescence Oligonucleotide Array Sequence Analysis Genetics Radiation Thyroid Chromosome Mapping Infant DNA Neoplasm Sequence Analysis DNA medicine.disease Molecular biology medicine.anatomical_structure chemistry Chernobyl Nuclear Accident Child Preschool Mutation Female Radioactive Hazard Release Ukraine Sequence Alignment DNA SNP array Comparative genomic hybridization Power Plants |
| Zdroj: | Radiation research. 166(3) |
| ISSN: | 0033-7587 |
| Popis: | Genetic gains and losses resulting from DNA strand breakage by ionizing radiation have been demonstrated in vitro and suspected in radiation-associated thyroid cancer. We hypothesized that copy number deviations might be more prevalent, and/or occur in genomic patterns, in tumors associated with presumptive DNA strand breakage from radiation exposure than in their spontaneous counterparts. We used cDNA microarray-based comparative genome hybridization to obtain genome-wide, high-resolution copy number profiles at 14,573 genomic loci in 23 post-Chernobyl and 20 spontaneous thyroid cancers. The prevalence of DNA gains in tumors from cases in exposed individuals was two- to fourfold higher than for cases in unexposed individuals and up to 10-fold higher for the subset of recurrent gains. DNA losses for all cases were low and more prevalent in spontaneous cases. We identified unique patterns of copy variation (mostly gains) that depended on a history of radiation exposure. Exposed cases, especially the young, harbored more recurrent gains that covered more of the genome. The largest regions, spanning 1.2 to 4.9 Mbp, were located at 1p36.32-.33, 2p23.2-.3, 3p21.1-.31, 6p22.1-.2, 7q36.1, 8q24.3, 9q34.11, 9q34.3, 11p15.5, 11q13.2-12.3, 14q32.33, 16p13.3, 16p11.2, 16q21-q12.2, 17q25.1, 19p13.31-qter, 22q11.21 and 22q13.2. Copy number changes, particularly gains, in post-Chernobyl thyroid cancer are influenced by radiation exposure and age at exposure, in addition to the neoplastic process. |
| Databáze: | OpenAIRE |
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